货号：MABF24

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho ZAP-70 (Tyr492) Antibody, clone EP2291Y, Rabbit | MABF24 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: The antibody only detects ZAP-70 phosphorylated on Tyr492. View All» Molecular Weight: ~70 kDa observed View All» Epitope: Tyr492 View All» Immunogen: Phospho-synthetic peptide corresponding to human ZAP-70 phosphorylated on Tyr492. View All» Modifications: Phosphorylation View All» Clone: EP2291Y View All» Isotype: IgG View All» Background Information: Zap-70, a Syk-family protein tyrosine kinase, plays a critical role in mediating T cell signal transduction in response to T cell receptor (TCR) activation (1). TCR-mediated activation of the Src-family kinases, Lck and Fyn, results in tyrosine phosphorylation of the TCR zeta and CD3 chains. These domains serve as targets for binding of ZAP-70 via its tandem SH2 domains. This binding correlates with activation of ZAP-70, a critical event in T cell activation (2). Following TCR engagement, ZAP-70 is phosphorylated on several tyrosine residues, presumably by two mechanisms: an autophosphorylation and a trans-phosphorylation by the Src-family tyrosine kinase, Lck. Lck phosphorylates Tyrosine 492 and 493 located at the activation loop of the catalytic domain of ZAP-70, leading to its activation and increased autophosphorylation . View All» Species Reactivity: Human View All» Species Reactivity Note: Proven to react with human. View All» Application Notes: Immunoprecipitation Analysis: A 1:30 dilution from a previous lot detected ZAP-70 in IP. View All» Control: Pervanadate treated and untreated Jurkat cell lysate. View All» Quality Assurance: Evaluated by Western Blot in pervanadate treated and untreated Jurkat cell lysate.Western Blot Analysis: A 1:10,000 – 1:20,000 dilution of this antibody detected ZAP-70 in pervanadate treated and untreated Jurkat cell lysate. View All» Purification Method: Unpurified View All» Presentation: Rabbit monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P43403 View All» Entrez Gene Number: NP_001070.2 View All» Gene Symbol:

• STD

• SRK

• ZAP-70

• FLJ17670

• TZK

• FLJ17679

• EC 2.7.10.2
  View All» Alternate Names:
  • zeta-chain (TCR) associated protein kinase 70kDa

  • Syk-related tyrosine kinase

  • 70 kDa zeta-associated protein

  • zeta-chain (TCR) associated protein kinase (70 kD)

  • zeta-chain associated protein kinase, 70kD

  • tyrosine-protein kinase ZAP-70
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Immunoprecipitation
      View All» Entrez Gene Summary: This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene. View All» UniProt Summary: FUNCTION: Plays a role in T-cell development and lymphocyte activation. Essential for TCR-mediated IL-2 production. Isoform 1 induces TCR-mediated signal transduction, isoform 2 does not.CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.SUBUNIT STRUCTURE: Interacts with SLA2 when it is phosphorylated. Interacts with CD3Z and with phosphorylated NFAM1. Interacts with CBLB By similarity. Interacts with CBL and SLA when it is phosphorylated. The association with SLA (or SLA2) and CBL probably leads to its destruction. Interacts with SHB. Interacts with DEF6. Interacts (via SH2 domains) with RHOH By similarity. Interacts with FCRL3. SUBCELLULAR LOCATION: Cytoplasm By similarity. Cell membrane By similarity. Note: After antigen stimulation, isoform 1 concentrates at the immunological synapse and isoform 2 remains cytoplasmic. Co-localizes together with RHOH in the immunological synapse. RHOH is required for its proper localization to the cell membrane and cytoskeleton fractions in the thymocytes By similarity.TISSUE SPECIFICITY: Expressed in T- and natural killer cells. DOMAIN: The SH2 domains bind to the phosphorylated tyrosine-based activation motif (TAM) of CD3Z and the non-canonical phosphorylated tyrosine-based activation motif (TAM) of RHOH By similarity.PTM: Phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation. Tyr-319 phosphorylation is essential for full activity. INVOLVEMENT IN DISEASE: Defects in ZAP70 are the cause of selective T-cell defect (STD) [MIM:176947]. STD is an autosomal recessive form of severe combined immunodeficiency characterized by a selective absence of CD8-type T-cells. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.Contains 1 protein kinase domain.Contains 2 SH2 domains. View All» Product Name: Anti-phospho ZAP-70 (Tyr492), clone EP2291Y, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Unpurified View All» Host: Rabbit View All»
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