货号：MABS175

品牌：Merck Millipore

规格：EA

目录价：￥3240.00

市场价格：￥2754.00

会员价格：￥2592.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho KSR1 (Ser404) Antibody, clone EP2358Y, Rabbit | MABS175 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes KSR1 phosphorylated at Ser404. View All» Molecular Weight: ~110 observed View All» Epitope: Phosphorylated Ser404 View All» Immunogen: Synthetic phospho-peptide corresponding to human KSR1 phosphorylated Ser404. View All» Modifications: Phosphorylation View All» Clone: EP2358Y View All» Isotype: IgG View All» Background Information: Kinase suppressor of Ras (KSR) is a conserved component of the Ras pathway that interacts directly with MEK and MAPK. It has been shown that KSR1 translocates from the cytoplasm to the cell surface in response to growth factor treatment and that this process is regulated by MARK3 (1). MARK3 phosphorylates serine 309 and serine 404 on KSR1. While, PPP2CA dephosphorylates serine 404 on KSR1. MARK3 seems to be a positive regulator of the beta-catenin pathway and an inhibitor of the JNK pathway. These findings show that MARK3, a regulator of polarity, is also a modulator of Wnt-beta-catenin signaling, indicating a link between two important developmental pathways (2). View All» Species Reactivity: Human View All» Species Reactivity Note: Demonstrated to react with human. View All» Application Notes: Immunocytochemistry Analysis: 1:100-1:250 dilution from a representative lot detected KSR1 in Sh-SY-5Y cells. View All» Control: Calyculin A untreated and treated HeLa cell lysates View All» Quality Assurance: Evaluated by Western Blot in Calyculin A untreated and treated HeLa cell lysates.Western Blot Analysis: 1:2,000 dilution of this antibody detected KSR1 in Calyculin A untreated and treated HeLa cell lysates. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: Q8IVT5 View All» Entrez Gene Number: Q8IVT5 View All» Gene Symbol:

• KSR

• RSU2

• OTTHUMP00000162931
  View All» Alternate Names:
  • kinase suppressor of ras 1

  • kinase suppressor of ras
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Immunocytochemistry
      View All» UniProt Summary: FUNTION: Location-regulated scaffolding protein connecting MEK to RAF. Promotes MEK and RAF phosphorylation and activity through assembly of an activated signaling complex. By itself, it has no demonstrated kinase activity.SUBUNIT STRUCTURE: Interacts with HSPCA/HSP90, YWHAB/14-3-3, CDC37, MAP2K/MEK, MARK3, PPP2R1A and PPP2CA. Also interacts with RAF and MAPK/ERK, in a Ras-dependent manner By similarity. The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization.SUBCELLULAR LOCATION: Cytoplasm By similarity. Membrane; Peripheral membrane protein By similarity. Note: In unstimulated cells, where the phosphorylated form is bound to a 14-3-3 protein, sequestration in the cytoplasm occurs. Following growth factor treatment, the protein is free for membrane translocation, and it moves from the cytoplasm to the cell periphery By similarity.PTM: Phosphorylated on Ser-309 and, to a higher extent, on Ser-404 by MARK3. Dephosphorylated on Ser-404 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated By similarity. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.Contains 1 phorbol-ester/DAG-type zinc finger.Contains 1 protein kinase domain. View All» Product Name: Anti-phospho KSR1 (Ser404), clone EP2358Y, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Diluted Culture Supernatant View All» Host: Rabbit View All»
      © 金山科研平台是专业的授权总代理区域代理经销平台。
      © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
      © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
      © 本文地址：https://www.16ao.com/thread-133167.htm
      © Merck Millipore代理MABS175 Anti-phospho-KSR1 (Ser404) clone EP2358Y，产品报价联系微信jinshanbio