货号：MABT145

品牌：Merck Millipore

规格：

目录价：￥3990.00

市场价格：￥3391.50

会员价格：￥3192.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Calreticulin Antibody, clone EPR3924, Rabbit | MABT145 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Molecular Weight: ~55 kDa observed View All» Immunogen: Synthetic peptide corresponding to human Calreticulin. View All» Clone: EPR3924 View All» Isotype: IgG View All» Background Information: Calreticulin is a molecular Ca+ binding chaperone promoting folding, oligomeric assembly, and quality control in ER. Calreticulin is a highly versatile lectin-like chaperone, and it participates during the synthesis of a variety of molecules, including ion channels, surface receptors, integrins and transporters (1). Calreticulin indeed provides up to 45% of the Ca2+-buffering capacity for a pool of the IP3-sensitive Ca2+ inside the ER (2). Calreticulin also functions in cell adhesion, integrin-dependent Ca2+ signaling and steroid-sensitive gene expression (1). View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Demonstrated to react with human, mouse, and rat. View All» Application Notes: Immunoprecipitation Analysis: 1:10 dilution from a representative lot was used in IP.Flow Cytometry Analysis: 1:10 dilution from a representative lot was used in FC.Immunohistochemistry Analysis: 1:250-1:500 dilution from a representative lot detected Calreticulin in human kidney tissue.Immunocytochemistry Analysis: 1:100-250 dilution from a representative lot was used to detect Calreticulin in HeLa cells. View All» Control: SH-SY5Y, HL-60, HepG2, HeLa, fetal kidney, and fetal brain lysates View All» Quality Assurance: Evaluated by Western Blot in SH-SY5Y, HL-60, HepG2, HeLa, fetal kidney, and fetal brain lysatesWestern Blot Analysis: 1:1,000-10,000 dilution of this antibody detected Calreticulin in SH-SY5Y, HL-60, HepG2, HeLa, fetal kidney, and fetal brain lysates. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below-20°C may cause glycerol containing Solutions to become frozen during storage. View All» UniProt Number: P27797 View All» Gene Symbol:
  • SSA

  • FLJ26680

  • cC1qR

  • RO

  • CRT

  • grp60

  • ERp60

  • HACBP

  • CRP55

  • CRTC
    View All» Alternate Names:
    • calreticulin

    • Endoplasmic reticulum resident protein 60

    • Sicca syndrome antigen A (autoantigen Ro; calreticulin)

    • calregulin

    • autoantigen Ro

    • Calregulin
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunocytochemistry

      • Immunohistochemistry (Paraffin)

      • Flow Cytometry

      • Immunoprecipitation
        View All» UniProt Summary: FUNCTION: Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. SUBUNIT STRUCTURE: Monomer. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interacts with PDIA3/ERp57 By similarity. Interacts with NR3C1 and TRIM21. SUBCELLULAR LOCATION: Endoplasmic reticulum lumen. Cytoplasm › cytosol. Secreted › extracellular space › extracellular matrix. Cell surface. Note: Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes. DOMAIN: Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.The interaction with glycans occurs through a binding site in the globular lectin domain.The zinc binding sites are localized to the N-domain.Associates with PDIA3 through the tip of the extended arm formed by the P-domain.SEQUENCE SIMILARITIES: Belongs to the calreticulin family.CAUTION: Was originally (Ref.1) thought to be the 52 kDa Ro autoantigen.MASS SPECTROMETRY: Molecular mass is 46879 Da from positions 18 - 417. Determined by MALDI. View All» Product Name: Anti-Calreticulin, clone EPR3924, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Diluted Culture Supernatant View All» Host: Rabbit View All»
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