货号：MABT146

品牌：Merck Millipore

规格：

目录价：￥3990.00

市场价格：￥3391.50

会员价格：￥3192.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-CD97 Antibody, clone EPR4427, Rabbit | MABT146 View All» Promotional Text: Special Offer on Antibodies! Click Here! View All» Molecular Weight: ~74-89 kDa observed View All» Epitope: Extracellular domain View All» Immunogen: Synthetic peptide corresponding to human CD97 at the extracellular domain View All» Clone: EPR4427 View All» Isotype: IgG View All» Background Information: CD97 is a member of the EGF-TM7 family of class II seven-span transmembrane (7-TM) molecules. It is a glycoprotein that is present on the surface of most activated leukocytes and spans the membrane seven times, which is a defining feature of G protein-coupled receptors. CD97 has an extended extracellular region with several N-terminal epidermal growth factor (EGF)-like domains, which mediate binding to its cellular ligand, decay accelerating factor (DAF, CD55), a regulatory protein of the complement cascade. The presence of structural features characteristic of extracellular matrix proteins and transmembrane proteins suggests that this protein is a receptor involved in both cell adhesion and signaling processes early after leukocyte activation. View All» Species Reactivity:

• Mouse

• Human
  View All» Species Reactivity Note: Demonstrated to react with Human and Mouse. View All» Application Notes: Immunocytochemistry Analysis: 1:250-1:500 dilution from a representative lot was used in IC. Immunoprecipitation Analysis: 1:10 to 1:100 dilution from a representative lot was used in IP.Immunohistochemistry Analysis: 1:250-1:500 dilution from a representative lot detected CD97 in human spleen tissue. View All» Control: Human tonsil, Jurkat, U937, and fetal thymus tissue lysates View All» Quality Assurance: Evaluated by Western Blot in human tonsil, Jurkat, U937, and fetal thymus tissue lysates.Western Blot Analysis: 1:1,000-1:10,000 dilution of this antibody detected CD97 in human tonsil, Jurkat, U937, and fetal thymus tissue lysates. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing 50% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P48960 View All» Entrez Gene Number: P48960 View All» Gene Symbol: TM7LN1 View All» Alternate Names:
  • CD97 molecule

  • CD97 antigen

  • Leukocyte antigen CD97

  • seven transmembrane helix receptor

  • seven-span transmembrane protein

  • seven-transmembrane, heterodimeric receptor associated with inflammation
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Immunocytochemistry

    • Immunohistochemistry (Paraffin)

    • Immunoprecipitation
      View All» UniProt Summary: FUNCTION: Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration By similarity.SUBUNIT STRUCTURE: Forms a heterodimer, consisting of a large extracellular region (alpha subunit) non-covalently linked to a seven-transmembrane moiety (beta subunit). Interacts with complement decay-accelerating factor (DAF). The largest isoform 1) interacts with chondroitin sulfate. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. CD97 antigen subunit alpha: Secreted › extracellular space. TISSUE SPECIFICITY: Broadly expressed, found on most hematopoietic cells, including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes. Expressed also abundantly by smooth muscle cells. Expressed in thyroid, colorectal, gastric, esophageal and pancreatic carcinomas too. Expression are increased under inflammatory conditions in the CNS of multiple sclerosis and in synovial tissue of patients with rheumatoid arthritis. Increased expression of CD97 in the synovium is accompagnied by detectable levels of soluble CD97 in the synovial fluid.INDUCTION: Rapid up-regulation during lymphocyte activation.DOMAIN: The first two EGF domains mediate the interaction with DAF. A third tandemly arranged EGF domain is necessary for the structural integrity of the binding region.Binding to chondroitin sulfate is mediated by the fourth EGF domain.PTM: Proteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane subunit By similarity.SEQUENCE SIMILARITIES: Belongs to the G-protein coupled receptor 2 family. LN-TM7 subfamily.Contains 5 EGF-like domains.Contains 1 GPS domain.SEQUENCE CAUTION: The sequence AAC27673.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAC06178.1 differs from that shown. Reason: Erroneous gene model prediction. View All» Product Name: Anti-CD97, clone EPR4427, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Host: Rabbit View All»
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