货号：MABT150

品牌：Merck Millipore

规格：

目录价：￥3978.00

市场价格：￥3381.30

会员价格：￥3182.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Cytokeratin 20 Antibody, clone EPR1622Y, Rabbit | MABT150 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Molecular Weight: ~46 kDa observed View All» Epitope: C-terminus View All» Immunogen: Synthetic peptide corresponding to human Cytokeratin 20 at the C-terminus. View All» Clone: EPR1622Y View All» Isotype: IgG View All» Background Information: Intermediate-sized filament (IF) protein designated cytokeratin (CK) 20 is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract (1). Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium (2). CK20 has recently been reported to be useful to distinguish between primary and metastatic lung adenocarcinoma. CK20 expression was significantly more prevalent in adenocarcinoma that originated in the GI tract than that of pulmonary or breast origin (3). View All» Species Reactivity:

• Human

• Rat

• Mouse
  View All» Species Reactivity Note: Demonstrated to react with human, mouse, and rat. View All» Application Notes: Immunohistochemistry Analysis: 1:100-1:250 dilution from a representative lot detected Cytokeratin 20 in human colon adenocarcinoma tissue.Immunocytochemistry Analysis: 1:100-1:250 dilution from a representative lot was used in IC. Immunoprecipitation Analysis: 1:40 dilution from a representative lot was used in IP. View All» Control: Human small intestine tissue lysate View All» Quality Assurance: Evaluated by Western Blot in human small intestine tissue lysate.Western Blot Analysis: 1:10,000 - 50,000 dilution of this antibody detected Cytokeratin 20 in human small intestine tissue lysate. View All» Purification Method: Unpurified View All» Presentation: Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P35900 View All» Entrez Gene Number: P35900 View All» Gene Symbol:
  • keratin 20

  • K20

  • CK20

  • MGC35423

  • Protein IT

  • CK-20

  • CD20

  • KRT21

  • keratin-20

  • cytokeratin 20

  • cytokeratin-20

  • Cytokeratin-20

  • Keratin-20
    View All» Alternate Names: keratin, type I cytoskeletal 20 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Immunocytochemistry

    • Immunohistochemistry (Paraffin)

    • Immunoprecipitation
      View All» UniProt Summary: Function Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine By similarity. Ref.4 Ref.8Subunit structure Heterotetramer of two type I and two type II keratins. Associates with KRT8.Subcellular location Cytoplasm Ref.6 Ref.7. Tissue specificity Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa. Ref.6 Ref.7Developmental stage First detected at embryonic week 8 in individual 'converted' simple epithelial cells of the developing intestinal mucosa. In later fetal stages, synthesis extends over most goblet cells and a variable number of villus enterocytes. In the developing gastric and intestinal mucosa, expressed in all enterocytes and goblet cells as well as certain 'low-differentiated' columnar cells, whereas the neuroendocrine and Paneth cells are negative. Ref.1Post-translational modification Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury By similarity.Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228. Ref.4Miscellaneous There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).Sequence similarities Belongs to the intermediate filament family. View All» Product Name: Anti-Cytokeratin 20, clone EPR1622Y, Rabbit Monoclonal View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Diluted Culture Supernatant View All» Host: Rabbit View All»
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