货号：MABT199

品牌：Merck Millipore

规格：100UL

目录价：￥4967.00

市场价格：￥4221.95

会员价格：￥3973.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Integrin Beta1 Antibody, clone 8E3 | MABT199 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes the N-terminus of the PSI domain of Integrin Beta1. Under native conditions, clone 8E3 is shown to recognize the unbent, monomeric ER form of Integrin Beta1 at the PSI domain; however, the epitope only becomes exposed in the Golgi form after denaturation. This observation suggests that the epitopes are present in monomeric beta 1-integrins, but become lost following assembly with alpha-integrin subunits (Tiwari, S., et al. (2011). J Cell Sci. 124(10):1672-1680.; Mould, A. P., et al. (2004). J Biol Chem. 280(56):4238-4226.) View All» Molecular Weight: ~100-150 kDa observed. The calculated molecular weight is 86 kDa; however, it is known to have multiple isoforms that range between ~87-91 kDa. This protein is highly glycosolated and depending on the cell line or tissue, it has been observed at ~100-150 kDa (Bridger, P. S., et al. (2008). Biol Reprod. 79(2):274-282.). View All» Epitope: N-terminus of the PSI domain View All» Immunogen: Purified Integrin Beta1 from HT-1080 cells. View All» Clone: 8E3 View All» Isotype: IgG1κ View All» Background Information: Integrin beta1 is a transmembrane glycoprotein that forms noncovalent complexes with various alpha integrin subunits to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metatastatic diffusion of tumor cells. Interactions between integrins and extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression. Phosphorylation of threonines 788 and 789 on integrin beta1 receptor may play a key role in cell-cycle dependent regulation. View All» Species Reactivity: Human View All» Species Reactivity Note: Demonstrated to react with Human. View All» Application Notes: Western Blot Analysis: A 1:100,000 dilution from a representative lot detected Integrin protein in WB under non-reducing conditions.Activity Assay Analysis: A representative lot from an independent laboratory stimulated cellular adhesion and increased ligand binding activity of Integrin Beta1 (Mould, A. P., et al. (2004). J Biol Chem. 280(56):4238-4226.).Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Integrin Beta1 in IP (Tiwari, S., et al. (2011). J Cell Sci. 124(10):1672-1680.). View All» Control: HT-1080 cell lysate View All» Quality Assurance: Evaluated by Western Blot in HT-1080 cell lysate.Western Blot Analysis: A 1:2,000 dilution of this antibody detected Integrin Beta1 in 10 µg of HT-1080 cell lysate. View All» Purification Method: Protein G purified View All» Presentation: Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: P05556 View All» Entrez Gene Number: NP_002202 View All» Gene Symbol:

• ITGB

• FNRB

• MDF2

• MSK12
  View All» Alternate Names:
  • Integrin beta-1

  • Fibronectin receptor subunit beta

  • VLA-4 subunit beta

  • CD29
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Activity Assay

    • Immunoprecipitation
      View All» Product Name: Anti-Integrin Beta1, clone 8E3 View All» Concentration: 0.5 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Purified View All» Host: Mouse View All»
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