货号:MAC111
品牌:Merck Millipore
规格:50Ug
目录价:¥3516.00
市场价格:¥2988.60
会员价格:¥2812.80
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Description:
Anti-GST•Tag™ fusion protein Antibody | MAC111
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Promotional Text:
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Molecular Weight:
~45 kDa observed. The molecular weight of the GST•Tag fusion protein is not ~45 kDa. The Posi-Tag Epitope Tag Control, which contains the fusion protein, is observed at ~45 kDa in Western Blot.
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Immunogen:
Gluththione-S-transferase (GST) domain from Schistosoma Japonicum
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Clone:
8-326.6.1
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Isotype:
IgG1κ
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Background Information:
GST (glutathione-S-transferase) represents a major group of detoxification enzymes, which are known to exist as multiple cytosolic and membrane-bound isoenzymes, with distinct catalytic and noncatalytic binding properties. Whereas the cytosolic enzymes are encoded by at least five distantly related gene families, the membrane-bound enzymes are encoded by single genes. GST is regulated and induced by a diverse range of xenobiotics. Many, but not all, inducers of GST, act through either the antioxidant-responsive element (ARE), the GST P enhancer 1(GPE), the xenobiotic-responsive element (XRE), or the glucocorticoid-responsive element (GRE). Barbiturates may transcriptionally activate GST through a Barbie box element. Many of the compounds that induce GST are also GST substrates, or are metabolized by cytochrome P-450 monooxygenases into compounds that can serve as GST substrates. Because the expression levels of GST play a critical role in determining the sensitivity of cells to a broad range of toxic chemicals, GST is suggested to contribute to resistance against carcinogens, antitumor drugs, environmental pollutants, and products of oxidative stress.
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Application Notes:
Immunocytochemistry Analysis: A representative lot detected GST•Tag fusion protein in transfected COS-1 cells.Immunoprecipitation Analysis: A representative lot immunoprecipitated GST•Tag fusion protein sequence in IP.ELISA Analysis: A representative lot detected GST•Tag protein sequence in ELISA.
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Control:
Posi-Tag Epitope Tag Control
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Quality Assurance:
Evaluated by Western Blot in Posi-Tag Epitope Tag Control.Western Blot Analysis: This antibody detected GST•Tag fusion protein in 5 µL of Posi-Tag Epitope Tag Control.
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Purification Method:
Protein G purified
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Presentation:
Purified mouse monoclonal IgG1κ cultured supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
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Storage Conditions:
Stable for 1 year at 2-8°C from date of receipt.
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UniProt Number:
P08515
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Alternate Names:
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Glutathione S-transferase class-mu 26 kDa isozyme
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GST 26
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Sj26 antigen
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SjGST
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Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Key Applications:
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Western Blotting
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Immunocytochemistry
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Immunoprecipitation
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ELISA
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Product Name:
Anti-GST•Tag™ fusion protein
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Concentration:
1 mg/mL
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Antibody Type:
Monoclonal Antibody
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Qty/Pk:
50 μg
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Format:
Purified
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Host:
Mouse
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