货号：05-1579

品牌：Merck Millipore

规格：100Ug

目录价：￥5053.00

市场价格：￥4295.05

会员价格：￥4042.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Axin-1 Antibody, clone A5 | 05-1579 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes the N-terminus of Axin-1. View All» Molecular Weight: ~ 97 kDa View All» Epitope: N-terminus View All» Immunogen: GST-tagged recombinant protein corresponding to the N-terminus of mouse Axin-1. View All» Clone: A5 View All» Isotype: IgG2aκ View All» Background Information: Axin-1 is constitutively expressed and acts as a negative regulator of Wnt expression. It has been demonstrated that mouse Wnt, Wnt3a, is secreted by multiple cell lines and can act and as a growth factor for stem cells. Secreted Wnt3a is highly hydrophobic because it is palmitoylated on an essential cysteine C77; the palmitoylated state of Wnt3a is essential for stem cell signaling. Incubation of stem cells with Wnt3a in tissue culture medium resulted in the increased number of mouse hematopoietic stem cells expressing "primitive" phenotype. View All» Species Reactivity:

• Human

• Mouse
  View All» Species Reactivity Note: Demonstrated to react with human. Predicted to react with mouse based on 100% sequence homology. View All» Control: SW-480 cell lysate View All» Quality Assurance: Evaluated by Western Blot using immunoprecipitated Axin-1 in SW-480 cell lysate.Western Blot Analysis: 1 µg/mL of this antibody detected Axin-1 immunoprecipitated from 10 µg of SW-480 cell lysate. View All» Purification Method: Protein G purfied View All» Presentation: Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: O35625 View All» Entrez Gene Number: NP_033863.2 View All» Gene Symbol:
  • AXIN

  • hAxin
    View All» Alternate Names:
    • Axis inhibition protein 1

    • axin 1

    • axis inhibitor 1

    • fused, mouse, homolog of
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunoprecipitation

      • Immunocytochemistry
        View All» UniProt Summary: FUNCTION: Controls dorsoventral patterning via two opposing effects; down-regulates beta-catenin to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of beta-catenin and APC by GSK3B. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7 By similarity. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. SUBUNIT STRUCTURE: Homodimer. Interacts with GSK3B and beta-catenin. The interaction between axin and beta-catenin occurs via the armadillo repeats contained in beta-catenin. Ternary complex. Also binds to plakoglobin (gamma-catenin), APC, DVL and PP2A. Interacts with SMAD6, SMAD7 and RNF111 By similarity. Interacts with TP53 and HIPK2. Probably part of a complex consisting of TP53, HIPK2 and AXIN1. Interacts with DIXDC1; prevents interaction with MAP3K1. Interacts with MAP3K4, MDFI and MDFIC. Interacts with ANKRD6. Interacts with AIDA. SUBCELLULAR LOCATION: Cytoplasm By similarity. TISSUE SPECIFICITY: Expressed in embryonic stem cells.DEVELOPMENTAL STAGE: Widely expressed at E10.5 to E16.5 day.PTM: Probably phosphorylated by GSK3B and dephosphorylated by PP2A. SEQUENCE SIMILARITIES: Contains 1 DIX domain.Contains 1 RGS domain. View All» Product Name: Anti-Axin-1, clone A5 View All» Concentration: 1.0 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
        © 金山科研平台是专业的授权总代理区域代理经销平台。
        © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
        © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
        © 本文地址：https://www.16ao.com/thread-133669.htm
        © Merck Millipore代理05-1579 Anti-Axin-1 Antibody, clone A5;store at +2℃ to +8℃，产品报价联系微信jinshanbio