货号：05-180

品牌：Merck Millipore

规格：1EA

目录价：￥5141.24

市场价格：￥4370.05

会员价格：￥4112.99

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Cortactin (p80/85) Antibody, clone 4F11 | 05-180 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Recognizes Cortactin. View All» Molecular Weight: 80 kDa and 85 kDa View All» Immunogen: Mixture of affinity-purified tyrosine phosphoproteins from chick embryo fibroblasts expressing activated pp60src View All» Clone: 4F11 View All» Isotype: IgG1 View All» Background Information: Cortactin is present as a p80 and p85 kDa protein in many cell types. The protein was first identified as being a major substrate of the tyrosine kinase pp60SRC. The main structural feature of the protein is that at the N-terminus there are five and a half 37-amino acid repeats, these appear to be responsible for the actin-binding. This repeat region occupies about a third of the protein. The repeat region is followed by a helical region and then a proline rich region and finally at the C-terminus, an SH3 domain. The proline rich domain is thought to bind the SH2 domain of v-src the viral version of the normal c-src known to phosphorylate cortactin. Cortactin bundles microfilaments and this bundling is inhibited by tyrosine phosphorylation. One pathway that activates the pathway leading to tyrosine phosphorylation of cortactin results from a cell adhesion molecule in brain micro-vessel endothelial cells, another is the growth factor, but presumably the very many mechanisms for pp60src activation would also result in cortactin phosphorylation and subsequent loss of actin binding. View All» Species Reactivity:

• Avian

• Bovine

• Human

• Hamster

• Mouse

• Rat
  View All» Control: Positive Control Included: 3T3 Cell Lysate (12-305) Other controls: PC-12 cell lysate, HeLa whole cell lysate or A-431 whole cell lysate View All» Quality Assurance: routinely evaluated in mouse 3T3/A31 cell lysates or chicken fibroblast src-transformed cell lysate View All» Purification Method: Protein G purfied View All» Presentation: Protein G Purified immunoglobulin in Protein A Purified immunoglobulin in Purified immunoglobulin in 10mM PBS, pH 7.4 with 0.05% sodium azide. View All» Storage Conditions: Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. View All» UniProt Number: Q14247 View All» Entrez Gene Number:
  • NM_005231.2

  • NM_138565.1
    View All» Gene Symbol:
    • CTTN

    • EMS1

    • Amplaxin

    • FLJ34459

    • 1110020L01Rik
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunocytochemistry

      • Immunohistochemistry

      • Immunoprecipitation

      • Western Blotting
        View All» Entrez Gene Summary: This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene. View All» UniProt Summary: FUNCTION: SwissProt: Q14247 # May contribute to the organization of cell structure. The SH3 motif may function as a binding region to cytoskeleton. Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation.SIZE: 550 amino acids; 61636 Da SUBUNIT: Interacts with SHANK2 and SHANK3 via its SH2 domain. Also interacts with FGD1 (By similarity). Interacts with PLXDC2.SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell projection, lamellipodium (By similarity). Cell projection, ruffle (By similarity). Note=Associated with membrane ruffles and lamellipodia (By similarity).SIMILARITY: SwissProt: Q14247 ## Contains 7 cortactin repeats. & Contains 1 SH3 domain. View All» Brand Family: Upstate View All» Product Name: Anti-Cortactin (p80/85) Antibody, clone 4F11 | 05-180 View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 200 μg View All» Format: Purified View All» Host: Mouse View All»
        © 金山科研平台是专业的授权总代理区域代理经销平台。
        © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
        © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
        © 本文地址：https://www.16ao.com/thread-133684.htm
        © Merck Millipore代理05-180 Anti-Cortactin (p80/85) Antibody, clone 4F11，产品报价联系微信jinshanbio