货号：05-321MG

品牌：Merck Millipore

规格：1mg

目录价：￥56409.00

市场价格：￥47947.65

会员价格：￥45127.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Phosphotyrosine Antibody, clone 4G10® | 05-321MG View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: This antibody recognizes tyrosine-phosphorylated proteins from all species. View All» Molecular Weight: Dependent upon the molecular weight of the tyrosine phosphorylated protein being detected. View All» Immunogen: Phosphotyramine-KLH View All» Modifications: Phosphorylation View All» Clone: 4G10® View All» Isotype: IgG2bκ View All» Background Information: Some of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phosphotyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments. View All» Species Reactivity: All View All» Species Reactivity Note: All species View All» Application Notes: Immunoprecipitation: 2-4 μg of a previous lot immunoprecipitated quantitatively the phosphotyrosine containing proteins in the lysate of a confluent culture (10 cm dish) of cells expressing an activated tyrosine kinase. To preserve phosphotyrosine, add: 0.2 mM sodium orthovanadate to the lysis buffer. View All» Control: Untreated A-431 (negative control) and EGF treated A-431 (positive control) whole cell lysates.Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at -20°C. The lysate is stable for 6 months at -20°C. Before use, add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for immunoblot analysis. View All» Quality Assurance: Routinely evaluated on EGF-treated human A431 carcinoma cells.Western Blot Analysis: 0.5-2 μg/mL of this lot detected tyrosine-phosphorylated proteins in a modified RIPA lysate from EGF-treated human A431 carcinoma cells (Cohen, B., 1990; Druker, B. J., 1989; Kanakura, Y., 1991). View All» Purification Method: Protein G-Sepharose™ chromatography View All» Presentation: Purified mouse monoclonal IgG2bκ in buffer containing PBS, pH 7.4. Liquid at 2-8ºC. IgG2bκ mouse monoclonal antibody produced in vitro by mouse-mouse hybridoma 4G10® (FOX-NY [NS-1 derivative] myeloma x spleen cells). View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt.NOTE: DO NOT FREEZE. For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8ºC. Save the supernatant for application. View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:

• Immunoprecipitation

• Western Blotting
  View All» Brand Family: Upstate View All» Product Name: Anti-Phosphotyrosine Antibody, clone 4G10® | 05-321MG View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 1 mg View All» Format: Purified View All» Host: Mouse View All»
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