货号：05-361

品牌：Merck Millipore

规格：200Ug

目录价：￥5207.00

市场价格：￥4425.95

会员价格：￥4165.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-NFκB p52 Antibody | 05-361 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: This antibody recognizes the p52 subunit of NFκB and its p100 precursor. View All» Molecular Weight: 52 kDa; precursor at 100 kDa View All» Immunogen: GST-fusion protein corresponding to amino acids 1-444 of human NFκB p52 subunit (Schmidt, R., 1991;Bours, V., 1992). View All» Isotype: IgG2a View All» Background Information: The NFκB transcription factor was originally identified as a protein complex consisting of a DNA binding subunit and an associated protein. The DNA binding subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino terminus of a precursor designated p105. A cDNA has been isolated that encodes an alternative DNA binding subunit of NFκB. It is synthesized as a protein that is expressed in a variety of cell types and, like p105, undergoes cleavage to generate its NFκB subunit, in this case a protein designated p52 (previously referred to as p49). In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells. View All» Species Reactivity: Human View All» Species Reactivity Note: Human. Does not cross-react with mouse or rat; other species cross-reactivity is unknown. View All» Application Notes: Immunoprecipitation: 4 µg of a previous lot immunoprecipitated NFκB p52 and its p100 precursor from 500 µg of HeLa nuclear extract cell lysate.Gel Shift Assay: A previous lot of this antibody used 2 µg per gel shift assay (Bours, V., 1993). View All» Control: Jurkat whole cell lysate, HeLa nuclear extract.Included Positive Antigen Control: Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 5-10 µL of extract and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels. View All» Quality Assurance: Routinely evaluated by immunoblotting in HeLa nuclear extract or in human Raji cell, but not in mouse 3T3/A31 cell and rat PC 12 cell lysatesWestern Blot Analysis: 0.5-2 µg/mL of this lot detected NFκB p52 and its p100 precursor in HeLa nuclear extract. A previous lot detected NFκB p52 and its p100 precursor in human Raji cell, but not in mouse 3T3/A31 cell and rat PC 12 cell lysates. Highly recommended for western blotting. View All» Purification Method: Protein G chromatography View All» Presentation: Purified mouse monoclonal IgG2a in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: Q00653 View All» Entrez Gene Number: NM_019408 View All» Gene Symbol:

• NFKB2

• LYT10

• H2TF1

• LYT-10

• Lyt10
  View All» Alternate Names:
  • DNA-binding factor KBF2

  • Lymphocyte translocation chromosome 10

  • Oncogene Lyt-10

  • nuclear factor of kappa light chain gene enhancer in B-cells 2

  • nuclear factor of kappa light polypeptide gene enhancer in B-cells 2

  • nuclear factor of kappa light polypeptide gene enhancer in B-cells 2(p49/p100)
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Electrophoretic Mobility Shift Assay

    • Immunoprecipitation

    • Western Blotting
      View All» Entrez Gene Summary: NFKB has been detected in numerous cell types that express cytokines, chemokines, growth factors, cell adhesion molecules, and some acute phase proteins in health and in various disease states. NFKB is activated by a wide variety of stimuli such as cytokines, oxidant-free radicals, inhaled particles, ultraviolet irradiation, and bacterial or viral products. Inappropriate activation of NF-kappa-B has been linked to inflammatory events associated with autoimmune arthritis, asthma, septic shock, lung fibrosis, glomerulonephritis, atherosclerosis, and AIDS. In contrast, complete and persistent inhibition of NF-kappa-B has been linked directly to apoptosis, inappropriate immune cell development, and delayed cell growth. For reviews, see Chen et al. (1999) [PubMed 9895331] and Baldwin (1996) [PubMed 8717528].[supplied by OMIM] . View All» UniProt Summary: FUNCTION: SwissProt: Q00653 # Appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65.SIZE: 900 amino acids; 96749 Da SUBUNIT: Active NF-kappa-B is a heterodimer of an about 52 kDa DNA-binding subunit and the weak DNA-binding subunit p65. Two heterodimers might form a labile tetramer.SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).DOMAIN: SwissProt: Q00653 The C-terminus of p100 might be involved in cytoplasmic retention, inhibition of DNA-binding by p52 homodimers, and/or transcription activation (By similarity). & The glycine-rich region (GRR) appears to be a critical element in the generation of p52.PTM: While translation occurs, the particular unfolded structure after the GRR repeat promotes the generation of p52 making it an acceptable substrate for the proteasome. This process is known as cotranslational processing. The processed form is active and the unprocessed form acts as an inhibitor (I kappa B-like), being able to form cytosolic complexes with NF-kappa B, trapping it in the cytoplasm. Complete folding of the region downstream of the GRR repeat precludes processing. & Subsequent to MAP3K14-dependent serine phosphorylation, p100 polyubiquitination occurs then triggering its proteasome-dependent processing. & Constitutive processing is tightly suppressed by its C- terminal processing inhibitory domain, named PID, which contains the death domain.DISEASE: SwissProt: Q00653 # A chromosomal aberration involving NFKB2 is found in a case of B-cell non Hodgkin lymphoma (B-NHL). Translocation t(10;14)(q24;q32) with IGHA1. The resulting oncogene is also called Lyt-10C alpha variant. & A chromosomal aberration involving NFKB2 is found in a cutaneous T-cell leukemia (C-TCL) cell line. This rearrangement produces the p80HT gene which encodes for a truncated 80 kDa protein (p80HT). & In B-cell leukemia (B-CLL) cell line, LB40 and EB308, can be found after heterogeneous chromosomal aberrations, such as internal deletions.SIMILARITY: Contains 7 ANK repeats. & Contains 1 death domain. & Contains 1 RHD (Rel-like) domain. View All» Brand Family: Upstate View All» Product Name: Anti-NFκB p52 Antibody | 05-361 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 200 µg View All» Format: Purified View All» Host: Mouse View All»
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