货号：05-373

品牌：Merck Millipore

规格：200Ug

目录价：￥5053.00

市场价格：￥4295.05

会员价格：￥4042.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Cyclin B1 Antibody, clone GNS3 (8A5D12) | 05-373 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: This antibody is specific for human cyclin B1, Mr 58 kDa. Does not cross-react with cyclin A or cyclin D. View All» Molecular Weight: 58 kDa View All» Immunogen: Recombinant human cyclin B1 View All» Clone: GNS3 (8A5D12) View All» Isotype: mixed IgGs View All» Background Information: Regulation of cell cycle progression in eukaryotic cells depends on the expression of cyclin proteins. These proteins are the regulatory subunits of the cell cycle-dependent kinases that are responsible for the phosphorylation of several cellular targets. The B cyclins, when combined with p34cdc2, a catalytic subunit of the cell cycle dependent kinase, form an enzyme that is active at the transition to the G2 phase, attaining maximum level at M phase. At that phase, the complex associates with condensed chromosomes in prophase and metaphase and is degraded at the metaphase/anaphase transition. Anti-Cyclin B1 may be used for the detection of cyclin B1 expression and for measuring relative differences in cyclin B1 level as a function of cell cycle phase. View All» Species Reactivity:

• Human

• Mouse
  View All» Species Reactivity Note: In addition to human, weak species cross-reactivity was observed with mouse. View All» Application Notes: Immunoprecipitation: 2 μg of a previous lot immunoprecipitated human cyclin B1 and cdc2 kinase from 500 μg of A431 RIPA lysate as determined by a subsequent immunoblot of the immunoprecipitate using anti-cdk1/cdc2 (PSTAIR), (Catalog # 06-923). View All» Control: A431 cellsIncluded Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. View All» Quality Assurance: Routinely evaluated by western blot analysis on RIPA lysate from human A431 carcinoma cells.Western Blot Analysis: 0.5-2 μg/mL of this lot detected cyclin B1 in RIPA lysates from human A431 carcinoma cells. View All» Purification Method: Protein G chromatography View All» Presentation: Protein G purified mouse IgGs in storage buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Frozen at -20°C. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: P14635 View All» Entrez Gene Number: NM_031966 View All» Gene Symbol:
  • CCNB1

  • CCNB
    View All» Alternate Names:
    • G2/mitotic-specific cyclin B1

    • cyclin B1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunoprecipitation

      • Western Blotting
        View All» Entrez Gene Summary: The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. View All» UniProt Summary: FUNCTION: SwissProt: P14635 # Essential for the control of the cell cycle at the G2/M (mitosis) transition.SIZE: 433 amino acids; 48337 Da SUBUNIT: Interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Binds HEI10. Interacts with catalytically active RALBP1 and CDC2 during mitosis to form an endocytotic complex during interphase.SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Centrosome.DEVELOPMENTAL STAGE: Accumulates steadily during G2 and is abruptly destroyed at mitosis.PTM: Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.SIMILARITY: SwissProt: P14635 ## Belongs to the cyclin family. Cyclin AB subfamily. View All» Brand Family: Upstate View All» Product Name: Anti-Cyclin B1 Antibody, clone GNS3 (8A5D12) | 05-373 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 200 µg View All» Format: Purified View All» Host: Mouse View All»
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