货号：05-413

品牌：Merck Millipore

规格：200Ug

目录价：￥5666.00

市场价格：￥4816.10

会员价格：￥4532.80

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-GSK3 (Tyr279/Tyr216) Antibody, clone 5G-2F | 05-413 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: This antibody recognizes GSK3 is constitutively phosphorylated at this Tyrosine residue in resting cells. This immunogen is identical in 15 of 17 residues in human and rat GSK3α (Tyr279) and GSK3β (Tyr216). The phosphotyrosine residue can be removed from GSK3 by treatment with PTP1B. View All» Molecular Weight: 46 kDa (alpha) and 51 kDa (beta) View All» Epitope: a.a. 203-219 View All» Immunogen: Synthetic peptide (C-KQLLHGEPNVS [pY]ICSRY) corresponding to amino acids 203-219 derived from a region of the catalytic domain (between subdomain VII and VIII) of the Drosophila GSK3/shaggy enzyme coupled to ovalbumin. View All» Modifications: Phosphorylation View All» Clone: 5G-2F View All» Isotype: IgG1κ View All» Background Information: Glycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1 View All» Species Reactivity:

• Human

• Rat

• Mouse

• Dictyostelium

• Yeast (S. cerevisiae)
  View All» Species Reactivity Note: Mouse, rat, human, S. cerevisiae and Dictyostelium. Clone 5G-2F should cross-react with nearly all species because of the extremely high conservation of this region among the known enzymes of many different species. View All» Applications Not Recommended: Immunoprecipitation View All» Control: Jurkat cell lysate, or serum starve 3T3-L1 cells for 3 hours, then stimulate with 100 nM insulin or 100 ng/mL IGF 1 for 5 min.Included Positive Antigen Control: Catalog # 12-303, Jurkat lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels. View All» Quality Assurance: Routinely evaluated by immunoblot on human Jurkat cells, mouse 3T3/A31, human A431, or rat L6 cells.Western Blot Analysis: 0.5-2 µg/mL of this lot detected phospho-GSK3 in RIPA lysates from human Jurkat cells. Previous lots detected phospho-GSK3 in mouse 3T3/A31, human A431 lysates and rat L6 cells View All» Purification Method: Protein G chromatography View All» Presentation: Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Frozen at -20ºC, View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: P49840 View All» Entrez Gene Number: NP_063937 View All» Gene Symbol:
  • GSK3A

  • DKFZp686D0638

  • EC 2.7.11.26
    View All» Alternate Names:
    • GSK-3 alpha

    • glycogen synthase kinase 3 alpha
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: Glycogen synthase kinase 3-alpha (GSK3A; EC {2.7.1.37}) is a multifunctional protein serine kinase, homologous to Drosophila 'shaggy' (zeste-white3) and implicated in the control of several regulatory proteins including glycogen synthase (see GYS1, {138570}) and transcription factors (e.g., JUN, {165160}). It also plays a role in the WNT ({164820}) and PI3K (see PIK3CG, {601232}) signaling pathways (see review by {1:Ali et al. (2001)}).[supplied by OMIM] View All» UniProt Summary: FUNCTION: SwissProt: P49840 # Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN (By similarity).SIZE: 483 amino acids; 50981 Da SUBUNIT: Monomer (By similarity).SIMILARITY: SwissProt: P49840 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily. & Contains 1 protein kinase domain. View All» Brand Family: Upstate View All» Product Name: Anti-phospho-GSK3 (Tyr279/Tyr216) Antibody, clone 5G-2F | 05-413 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 200 µg View All» Format: Purified View All» Host: Mouse View All»
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