货号：05-457

品牌：Merck Millipore

规格：200Ug

目录价：￥6908.00

市场价格：￥5871.80

会员价格：￥5526.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Histone H1 Antibody, clone AE-4 | 05-457 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: This antibody recognizes histone H1, Mr 32-33 kDa. Doublet detected due to hyperphosphorylation of histone H1 subpopulation. Cross-reacts with other histone isoforms in the following order of signal strength: H3, H2B > H4 > H2A, as determined with recombinant protein. View All» Molecular Weight: 32-33 kDa (Histone H1); Doublet (hyperphosphorylated Histone H1); 17 kDa (other histone isoforms). View All» Immunogen: Nuclei from myeloid leukemia biopsy cells. Clone AE-4. View All» Clone: AE-4 View All» Isotype: IgG2a View All» Background Information: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. View All» Species Reactivity:

• Human

• Bovine

• Green Plants
  View All» Species Reactivity Note: Human and bovine. Predicted to stain nuclei from other animal and plant species. View All» Application Notes: Immunocytochemistry: 5-20 µg/mL of a previous lot showed positive nuclear immunostaining in A431 cells fixed with 4% paraformaldehyde and methanol.ELISA: Recommended. View All» Control: Nuclear extracts from NIH3T3 or HeLa cells. View All» Quality Assurance: Routinely evaluated by immunoblot on lysates from an acid extract of HeLa cells.Western Blot: 1-4 µg/mL of this lot detected histone H1 in lysates from an acid extract of HeLa cells. View All» Purification Method: Protein G purfied View All» Presentation: Purified mouse monoclonal IgG2a in buffer containing PBS, pH 7.2, with 0.1% sodium azide. Frozen solution. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: P10412 View All» Entrez Gene Number: NM_005322 View All» Gene Symbol:
  • HIST1H1E

  • H1F4

  • H1.4

  • dJ221C16.5

  • MGC116819
    View All» Alternate Names:
    • H1 histone family, member 4

    • Histone H1b

    • histone 1, H1e

    • histone cluster 1, H1e
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • ELISA

      • Immunocytochemistry

      • Western Blotting
        View All» Entrez Gene Summary: Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. View All» UniProt Summary: FUNCTION: SwissProt: P10412 # Histones H1 are necessary for the condensation of nucleosome chains into higher order structures.SIZE: 219 amino acids; 21865 Da SUBCELLULAR LOCATION: Nucleus.SIMILARITY: SwissProt: P10412 ## Belongs to the histone H1/H5 family. MISCELLANEOUS: This variant accounts for 60% of histone H1. View All» Brand Family: Upstate View All» Product Name: Anti-Histone H1 Antibody, clone AE-4 | 05-457 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 200 µg View All» Format: Purified View All» Host: Mouse View All»
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