货号：05-535

品牌：Merck Millipore

规格：1EA

目录价：￥5181.05

市场价格：￥4403.89

会员价格：￥4144.84

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-IKKβ Antibody, clone 10AG2 | 05-535 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: This antibody recognizes IKKβ/IKK2, Mr 87 kDa. View All» Molecular Weight: 87 kDa View All» Immunogen: His-tagged, full length human IKKβ/IKK2. Clone: 10AG2. View All» Clone: 10AG2 View All» Isotype: IgG1 View All» Background Information: IKK beta (I-Kappa-B kinase-beta) is a member of the IKK complex which is composed of IKK alpha, IKK beta, IKK gamma and IKAP. Phosphorylation of I-Kappa-B on a serine residue by the IKK complex frees NF-kB from I-Kappa-B and marks it for degradation via ubiquination. IKK beta has been shown to activate NF-kB and phosphorylate IKB alpha and beta. Phosphorylation of 2 sites at the activation loop of IKK beta is essential for activation of IKK by TNF and IL1. Once activated, IKK beta autophosphorylates which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response. Additionally, IKK beta activity can also be regulated by MEKK-1. View All» Species Reactivity:

• Human

• Rat

• Mouse
  View All» Species Reactivity Note: Proven to react with human, mouse and rat. Other species not tested. View All» Control: Jurkat cell lysate and human cervical carcinoma.Included Positive Antigen Control: Catalog # 12-303, Jurkat cell extract. Use 20 µg per lane for minigels. View All» Quality Assurance: Routinely evaluated by western blot on human Jurkat RIPA cell lysate, human A431, HeLa nuclear extract, mouse 3T3/A31 or rat PC-12 RIPA cell lysates.Western Blot Analysis:0.5-2 µg/mL of this lot detected IKK/IKK2 in human HeLa cell lysate. A previous lot was tested in human Jurkat RIPA cell lysate, A431, extract, mouse 3T3/A31 and rat PC-12 RIPA cell lysates. View All» Purification Method: Protein G purfied View All» Presentation: Purified mouse monoclonal IgG1 in buffer containing PBS containing 0.05% BSA, with 0.05% sodium azide. Frozen solution. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. View All» UniProt Number: O14920 View All» Entrez Gene Number: NM_001556 View All» Gene Symbol:
  • IKBKB

  • IKK-beta

  • NFKBIKB

  • IKK2

  • FLJ40509

  • IKKB

  • MGC131801

  • IKK-B

  • IkBKB
    View All» Alternate Names:
    • I-kappa-B kinase 2

    • I-kappa-B-kinase beta

    • Nuclear factor NF-kappa-B inhibitor kinase beta

    • inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta

    • inhibitor of nuclear factor kappa B kinase beta subunit
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA (MIM 164014), or RELB (MIM 604758) to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008, or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM] View All» UniProt Summary: FUNCTION: SwissProt: O14920 # Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3 (By similarity).SIZE: 756 amino acids; 86564 Da SUBUNIT: Preferentially found as a heterodimer with IKK-alpha but also as a homodimer. Directly interacts with IKK-gamma/NEMO and TRPC4AP. The tripartite complex can also bind to MEKK1, MAP3K14/NIK, IKAP and IKB-alpha-p65-p50 complex. Phosphorylated IKB-alpha is further released from the complex. Found in a complex composed of NCOA2, NCOA3, IKKA, IKBKG and CREBBP. Interacts with SQSTM1 through PRKCZ or PRKCI. Forms an NGF-induced complex with IKBKB, PRKCI and TRAF6. May interact with MAVS/IPS1. Interacts with NALP2. Interacts with Yersinia yopJ.SUBCELLULAR LOCATION: Cytoplasm.TISSUE SPECIFICITY: Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.PTM: Upon cytokine stimulation, phosphorylated on Ser-177 and Ser- 181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response. & Yersinia yopJ may acetylate Ser/Thr residues, preventing phosphorylation and activation, which blocks the I-kappa-B signaling pathway.SIMILARITY: SwissProt: O14920 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. IkappaB kinase subfamily. & Contains 1 protein kinase domain. View All» Brand Family: Upstate View All» Product Name: Anti-IKKβ Antibody, clone 10AG2 | 05-535 View All» Concentration: 0.5 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µg View All» Format: Purified View All» Host: Mouse View All»
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