货号：05-649

品牌：Merck Millipore

规格：200Ug

目录价：￥5207.00

市场价格：￥4425.95

会员价格：￥4165.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Chk2 Antibody, clone 7 | 05-649 View All» Promotional Text: Special Shipping Offer on Antibodies 100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Chk2 View All» Molecular Weight: 67 kDa View All» Immunogen: Mixture of two GST fusion proteins corresponding to residues 1-223 and 217-543 of human Chk2 View All» Clone: clone 7 View All» Isotype: IgG1 View All» Background Information: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53. Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer. View All» Species Reactivity:

• Human

• Mouse
  View All» Species Reactivity Note: Human and mouse. Others not tested. Predicted to cross-react with rat based on sequence homology. View All» Application Notes: Immunoprecipitation: A previous lot of this antibody has been reported by an independent laboratory to immunoprecipitate Chk2 from HeLa cells. Immunocytochemistry: A previous lot of this antibody has been reported by an independent laboratory to detect Chk2 in HeLa cells. View All» Control: Jurkat cell lysate, HeLa cell lysate Included Positive Antigen Control: Catalog # 12-303, Jurkat Cell Lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. View All» Quality Assurance: Routinely evaluated by western blot on RIPA lysates from Jurkat cells, HeLa, 3T3/A31, or A431 cells. Western Blot Analysis: 0.1-1 μg/mL of this lot detected Chk2 in RIPA lysates from Jurkat cells. A previous lot detected Chk2 in lysates from HeLa, 3T3/A31, and A431 cells. View All» Purification Method: Protein G chromatography View All» Presentation: Protein G purified mouse IgG1 buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol. Liquid at -20°C. View All» Storage Conditions: Stable for 1 year at from date of receipt. View All» UniProt Number: O96017 View All» Entrez Gene Number: NP_001005735 View All» Gene Symbol:
  • CHEK2

  • HuCds1

  • CHK2

  • LFS2

  • RAD53

  • bA444G7

  • CDS1

  • OTTHUMP00000028871

  • PP1425

  • Cds1
    View All» Alternate Names:
    • CHK2 (checkpoint, S.pombe) homolog

    • CHK2 checkpoint homolog (S. pombe)

    • checkpoint-like protein CHK2

    • protein kinase CHK2

    • serine/threonine-protein kinase CHK2
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunoprecipitation

      • Immunocytochemistry
        View All» Entrez Gene Summary: In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene. View All» UniProt Summary: FUNCTION: SwissProt: O96017 # Regulates cell cycle checkpoints and apoptosis in response to DNA damage, particularly to DNA double-strand breaks. Inhibits CDC25C phosphatase by phosphorylation on 'Ser-216', preventing the entry into mitosis. May also play a role in meiosis. Regulates the TP53 tumor suppressor through phosphorylation at 'Thr-18' and 'Ser-20'. COFACTOR: Magnesium. SIZE: 543 amino acids; 60915 Da SUBCELLULAR LOCATION: Isoform 2: Nucleus. Note=Isoform 10 is present throughout the cell. & Isoform 4: Nucleus. & Isoform 7: Nucleus. & Isoform 9: Nucleus. & Isoform 12: Nucleus. TISSUE SPECIFICITY: High expression is found in testis, spleen, colon and peripheral blood leukocytes. Low expression is found in other tissues. DISEASE: SwissProt: O96017 # Defects in CHEK2 are associated with Li-Fraumeni syndrome 2 (LFS2) [MIM:609265]; a highly penetrant familial cancer phenotype usually associated with inherited mutations in p53/TP53. & Defects in CHEK2 are found in some patients with prostate cancer (CaP) [MIM:176807]. & Defects in CHEK2 are found in some patients with osteosarcoma (OSRC) [MIM:259500]. SIMILARITY: SwissProt: O96017 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CHK2 subfamily. & Contains 1 FHA domain. & Contains 1 protein kinase domain. View All» Brand Family: Upstate View All» Product Name: Anti-Chk2 Antibody, clone 7 | 05-649 View All» Concentration: 0.77 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 200 μg View All» Format: Purified View All» Host: Mouse View All»
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