货号：05-677

品牌：Merck Millipore

规格：100Ug

目录价：￥6895.00

市场价格：￥5860.75

会员价格：￥5516.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-Src (Tyr416) Antibody, clone 9A6 | 05-677 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Product Overview: Specific src isoform is unknown View All» Specificity: Recognizes phospho-Src, Mr 60kDa. The antibody does not crossreact with the nonphosphorylated form of Src or with unrelated phosphorylation sites. Predicted to recognize all Src family members phosphorylated at the tyrosine corresponding to Tyr416 of avian Src. View All» Molecular Weight: 60 kDa View All» Immunogen: KLH-conjugated, synthetic peptide containing phosphotyrosine in the sequence context corresponding to tyrosine 416 of avian Src. Clone 9A6. View All» Modifications: Phosphorylation View All» Clone: clone 9A6 View All» Isotype: IgG3 View All» Background Information: Src (also known as pp60src) is a non receptor Tyrosine Kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. There are two critical phosphorylation sites of tyrosine on Src, tyrosine 418 and tyrosine 529 (referring to human Src sequence). The tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418. This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated. View All» Species Reactivity:

• Human

• Mouse
  View All» Application Notes: Immunoprecipitation/Immunoblot Analysis: 4 μg/mL of a previous lot detected phospho-Src immunoprecipitated from 3T3 cell lysates with 10 μg of anti-Src (Catalog # 05-184), but not after phosphatase treatment with YOP. View All» Control: Chick embryo fibroblast (CEF) cells expressing human wild-type Src protein View All» Quality Assurance: Evaluated by immunoprecipitating Src from 3T3 cell lysates with anti-Src (Catalog #05-184), but not after phosphatase treatment with YOP.Src Autophosphorylation/Western Blotting Analysis: 0.5-2 μg/mL of this antibody strongly detected recombinant Src (Catalog # 14-326) after incubation with Mg/Mn/ATP. Phospho-specificity was confirmed by dephosphorylation of the Src protein with λ-phosphatase. View All» Purification Method: Thiophilic adsoption and size exclusion chromatography View All» Presentation: Purified mouse monoclonal IgG3 lyophilized from 1 mL 2x PBS / 0.09 % Na-azide / PEG and Sucrose. Reconstitute with 1 mL H2O (15 min, RT). Lyophilized. Store at -20ºC. View All» Storage Conditions: Stable for 1 year at from date of receipt. View All» UniProt Number: P12931 View All» Entrez Gene Number: NM_005417 View All» Gene Symbol:
  • SRC

  • c-src

  • p60-Src

  • SRC1

  • c-SRC

  • ASV

  • pp60c-src
    View All» Alternate Names:
    • proto-oncogene tyrosine-protein kinase SRC

    • protooncogene SRC, Rous sarcoma

    • tyrosine kinase pp60c-src

    • tyrosine-protein kinase SRC-1

    • v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog

    • v-src sarcoma (Schmidt-Ruppin A-2) viral oncogene homolog (avian)
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunoprecipitation
        View All» Entrez Gene Summary: This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. View All» UniProt Summary: SIZE: 536 amino acids; 59835 Da SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2.PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.SIMILARITY: SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain. View All» Brand Family: Upstate View All» Product Name: Anti-phospho-Src (Tyr416) Antibody, clone 9A6 | 05-677 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Mouse View All»
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