货号：05-767

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-CREB, clone NL904 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Recognizes CREB, Mr ~43kDa. A non-specific protein was also detected, Mr ~60 kDa. View All» Molecular Weight: Mr ~43 kDa View All» Epitope: a.a. 5-24 View All» Immunogen: peptide (C-SGAENQQSGDAAVTEAENQQ) corresponding to amino acids 5-24 of human CREB View All» Clone: NL904 View All» Isotype: IgG View All» Background Information: CREB is a basic leucine zipper transcription factor that functions in glucose homeostasis and stimulates the expression of numerous genes in response to growth factors, hormones, neurotransmitters, ion fluxes, stress signals, and has been implicated in memory because of its apparent role in synaptic plasticity. CREB functions either by itself or with related family members, which include CREB, CREM, and ATF-11. The CREB signaling pathway is activated by extracellular ligands that bind to cell surface receptors and relay their message through various intracellular second messengers resulting ultimately in the activation of nuclear CREB through phosphorylation of the transactivation domain at serine 1332. The classical model of CREB activation is through PKA. In the non-active state, PKA resides in the cytoplasm as an inactive heterotetramer of paired regulatory and catalytic subunits. Stimulation of cAMP causes the release of the catalytic subunits of PKA that passively diffuse into the nucleus and initiate gene expression by phosphorylating CREB at its transactivation domain on serine 1331. Target gene activation is terminated by the serine/threonine phosphatase PP-1 mediated dephosphorylation of CREB1. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Broad species cross reactivity is expected View All» Control: HeLa nuclear extract. Included Positive Antigen Control: Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 µL of extract and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced extract per lane for minigels. View All» Quality Assurance: Routinely evaluated by western blot on HeLa nuclear extract.Western Blot Analysis: A 1:2,000 to 1:10,000 dilution of this lot detected CREB on HeLa nuclear extract. View All» Purification Method: Protein A purfied View All» Presentation: Purified rabbit monoclonal IgG in buffer containing 0.1 M Tris, pH 7.4, 0.15 M NaCl, 5 mg/mL BSA and 0.05% sodium azide before the addition of glycerol to 30%. Liquid at -20°C. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P16220 View All» Entrez Gene Number: NM_134442 View All» Gene Symbol:
  • CREB1

  • CREB

  • MGC9284
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Immunoprecipitation

    • Western Blotting
      View All» Entrez Gene Summary: This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. View All» UniProt Summary: FUNCTION: SwissProt: P16220 # This protein binds the cAMP response element (CRE), a sequence present in many viral and cellular promoters. CREB stimulates transcription on binding to the CRE.SIZE: 341 amino acids; 36688 Da SUBUNIT: Binds DNA as a dimer. Interacts PPRC1 and with HTLV-1 Tax-1.SUBCELLULAR LOCATION: Nucleus.PTM: Stimulated by phosphorylation. Phosphorylation of both Ser- 133 and Ser-142 in the SCN regulates the activity of CREB and participates in circadian rhythm generation. Phosphorylation of Ser-133 allows CREBBP and EP300 binding (By similarity). Phosphorylated upon DNA damage, probably by ATM or ATR.SIMILARITY: SwissProt: P16220 ## Belongs to the bZIP family. & Contains 1 bZIP domain. & Contains 1 KID (kinase-inducible) domain. View All» Brand Family: Upstate View All» Product Name: Anti-CREB, clone NL904 View All» Concentration: Variable View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 200 μL View All» Format: Purified View All» Host: Rabbit View All»
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