货号：05-844

品牌：Merck Millipore

规格：200Ug

目录价：￥5053.00

市场价格：￥4295.05

会员价格：￥4042.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-PLK1 Antibody, clone 35-206 | 05-844 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: PLK1 View All» Molecular Weight: 64 kDa View All» Immunogen: 6His-tagged full length fusion protein corresponding to human polo-like kinase 1 (PLK1). Also called serine-threonine protein kinase PLK. View All» Clone: clone 35-206 View All» Isotype: IgG2b View All» Background Information: Polo-like Kinase 1 (PLK-1) is a serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the regulation of mitotic exit and cytokinesis. Expressed PLK-1 protein accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Human, mouse and rat. View All» Application Notes: Immunoprecipitation: An independent laboratory has reported that a previous lot of this antibody can be used to immunoprecipitate PLK1.Immunohisto/immunocytochemistry: An independent laboratory reported that 1 μg/mL of a previous lot of this antibody showed positive immunostaining for PLK1 in paraformaldehyde treated, Triton X-100 fixed cells. View All» Control: RIPA lysates from 3T3/A31 cellsIncluded Positive Antigen Control: Catalog # 12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. View All» Quality Assurance: Evaluated by western blot on RIPA lysates from 3T3/A31 cells.Western Blot Analysis: 0.5-2 μg/mL of this antibody detected PLK1 in RIPA lysates from 3T3/A31 cells. View All» Purification Method: Protein A purfied View All» Presentation: Protein A purified mouse IgG2b in buffer containing 0.07M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% sodium azide and 30% glycerol. Store at -20°C. View All» Storage Conditions: Stable for 1 year at from date of receipt. View All» UniProt Number: P53350 View All» Entrez Gene Number: NP_005021 View All» Gene Symbol:
  • PLK1

  • PLK

  • PLK-1

  • STPK13
    View All» Alternate Names:
    • Polo-like kinase 1

    • Serine/threonine-protein kinase 13

    • cell cycle regulated protein kinase

    • polo (Drosophia)-like kinase

    • polo like kinase

    • polo like kinase

    • polo-like kinase (Drosophila)

    • polo-like kinase 1 (Drosophila)
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunoprecipitation

      • Immunocytochemistry

      • Immunohistochemistry
        View All» UniProt Summary: FUNCTION: SwissProt: P53350 # May be required for cell division and may have a role during G1 or S phase.SIZE: 603 amino acids; 68255 Da SUBUNIT: Interacts with CEP170 and EVI5.SUBCELLULAR LOCATION: Nucleus.TISSUE SPECIFICITY: Placenta and colon.DEVELOPMENTAL STAGE: Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level following mitosis and throughout G1 phase, and then begins to accumulate again during S phase.PTM: Catalytic activity is enhanced by phosphorylation of Thr-210 and/or Ser-137. & Autophosphorylation and phosphorylation of Ser-137 are not significant events during activation of PLK1 in M phase.SIMILARITY: SwissProt: P53350 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily. & Contains 2 POLO box domains. & Contains 1 protein kinase domain. View All» Brand Family: Upstate View All» Product Name: Anti-PLK1 Antibody, clone 35-206 | 05-844 View All» Concentration: 1 mg/mL View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 200 μg View All» Format: Purified View All» Host: Mouse View All»
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