货号：05-858

品牌：Merck Millipore

规格：100UL

目录价：￥6742.00

市场价格：￥5730.70

会员价格：￥5393.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Histone H4 Antibody, pan, clone 62-141-13 | 05-858 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Product Overview: This antibody recognizes histone H4 independant of any post-translational modifications. View All» Specificity: Recognizes Histone H4, Mr 10 kDa. View All» Molecular Weight: 10 kDa View All» Epitope: a.a. 25-28 View All» Immunogen: KLH-conjugated, synthetic peptide corresponding to amino acids 17-28 of Histone H4. Epitope has been mapped to residues 25-28. View All» Isotype: IgG View All» Background Information: Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. View All» Species Reactivity:

• Human

• Chicken

• Vertebrates

• Yeast (S. cerevisiae)
  View All» Species Reactivity Note: Human, chicken and yeast. Broad species cross-reactivity expected based on sequence homology. View All» Application Notes: Epitope Mapping (using Luminex®): 1:250-1:4,000 serial dilutions of a previous lot were incubated with histone peptides containing various unmodified peptides spanning the amino-terminus of Histone H4 conjugated to Luminex® microspheres. The antibody recognized both modified and unmodified peptides (See Figure B Page 2).Chromatin Immunoprecipitation: An independent laboratory used a previous lot of this antibody in chromatin immunoprecipitation (Issac, C., 2006). View All» Control: Acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated). View All» Quality Assurance: Routinely evaluated by western blot.Western Blot Analysis: A 1:30,000-1:100,000 dilution of this lot detected unmodified recombinant Histone H4 protein (Catalog # 14-412) and Histone H4 in acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated) View All» Purification Method: Protein A purfied View All» Presentation: Purified rabbit monoclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: P62805 View All» Entrez Gene Number: NP_003529 View All» Gene Symbol:
  • HIST1H4A H4/J HIST1H4F HIST1H4L H4FN H4FA H4FH

  • HIST1H4H

  • H4F2

  • H4FM

  • H4/A

  • H4FK

  • H4FG

  • HIST2H4

  • H4FB

  • H4/K

  • HIST1H4I

  • H4/N

  • HIST1H4B

  • H4FE

  • H4/M

  • H4/a

  • HIST1H4E

  • HIST4H4

  • HIST2H4A

  • H4FC

  • H4FI

  • H4/H

  • HIST1H4C

  • H4/G

  • HIST1H4K

  • H4FJ

  • H4FD

  • H4/I

  • H4/B

  • H4/D

  • H4/C

  • HIST1H4J

  • HIST1H4D

  • H4/E
    View All» Alternate Names:
    • H4

    • Histone H4

    • H4 histone family, member A

    • histone 1, H4a

    • histone cluster 1, H4a
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Multiplexing

      • ChIP-seq
        View All» Entrez Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. View All» UniProt Summary: FUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.SIZE: 103 amino acids; 11367 Da SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.SUBCELLULAR LOCATION: Nucleus.PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family. View All» Brand Family: Upstate View All» Product Name: Anti-Histone H4 Antibody, pan, clone 62-141-13 | 05-858 View All» Concentration: Variable View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Purified View All» Host: Rabbit View All»
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