货号：05-952

品牌：Merck Millipore

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目录价：￥4977.00

市场价格：￥4230.45

会员价格：￥3981.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-RNA Polymerase II Antibody, CTD, clone 8WG16 | 05-952 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Recognizes RNA Polymerase II, Mr 220 kDa. View All» Molecular Weight: 220 kDa View All» Immunogen: RNA Polymerase II from wheat germ extract. Clone 8WG16. View All» Clone: 8WG16 View All» Background Information: As the study of the RNA polymerase II (RNAPII) transcription complex expands, many researchers are interested in using immunoblots to detect RNAPII in various protein preparations and column fractions. HeLa cell RNAPII contains 10 subunits, the largest possessing a heptapeptide repeat (PTSPSYS) in the C-terminal domain (CTD). This large subunit cycles, during the course of transcription, through nonphosphorylated (RNAPIIA) and hyperphosphorylated (RNAPIIO) forms. RNAPIIA associates with the basal transcription complex and becomes phosphorylated to the RNAPIIO form before the synthesis of the first phosphodiester bond. The timing of these events suggests that this phosphorylation event may function as a regulatory point, and there is considerable interest in identifying components of the transcription complex with CTD kinase activity View All» Species Reactivity:

• Human

• Yeast (S. cerevisiae)
  View All» Species Reactivity Note: Human and yeast. Broad species cross-reactivity is expected based on sequence conservation of immunogen. View All» Application Notes: Immunoprecipitation: Recommended by an independent laboratory.Immunocytochemistry: Recommended by an independent laboratory. View All» Control: HeLa nuclear extracts.Included Positive Antigen Control: Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 µL of extract and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced extract per lane for minigels. View All» Quality Assurance: Routinely evaluated by western blot.Western Blot Analysis: 1:500-1:2000 dilution of this lot detected RNA Polymerase II in HeLa nuclear extract. View All» Purification Method: Unpurified View All» Presentation: Mouse monoclonal in buffer containing 0.05% sodium azide and 30% glycerol. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P24928 View All» Entrez Gene Number: NP_000928 View All» Gene Symbol:
  • POLR2A

  • POLRA

  • RpIILS

  • POLR2

  • RPB1

  • MGC75453

  • hsRPB1

  • RPOL2

  • RPBh1

  • RPO2

  • hRPB220
    View All» Alternate Names:
    • DNA directed RNA polymerase II polypeptide A

    • DNA-directed RNA polymerase II largest subunit, RNA polymerase II 220kd subunit

    • DNA-directed RNA polymerase II subunit A

    • DNA-directed RNA polymerase III largest subunit

    • RNA polymerase II subunit B1

    • polymerase (RNA) II (DNA directed) polypeptide A (220kD)

    • polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunocytochemistry

      • Immunoprecipitation

      • Western Blotting
        View All» Entrez Gene Summary: This gene encodes the largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. The product of this gene contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, this subunit, in combination with several other polymerase subunits, forms the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. View All» UniProt Summary: FUNCTION: SwissProt: P24928 # DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Forms the polymerase active center together with the second largest subunit. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB1 is part of the core element with the central large cleft, the clamp element that moves to open and close the cleft and the jaws that are thought to grab the incoming DNA template. At the start of transcription, a single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol II. A bridging helix emanates from RPB1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol II by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for assembly of factors that regulate transcription initiation, elongation, termination and mRNA processing. View All» Brand Family: Upstate View All» Product Name: Anti-RNA Polymerase II Antibody, CTD, clone 8WG16 | 05-952 View All» Concentration: Variable View All» Antibody Type: Monoclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Ascites View All» Host: Mouse View All»
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