货号：06-1040

品牌：Merck Millipore

规格：

目录价：￥3724.00

市场价格：￥3165.40

会员价格：￥2979.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-RIG-I (N-Terminus) Antibody | 06-1040 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes RIG-I at the N-terminus. View All» Molecular Weight: ~102 kDa observed View All» Epitope: N-terminus View All» Immunogen: GST-tagged recombinant protein corresponding to the N-terminus of human RIG-I. View All» Background Information: Retinoic acid-inducible gene 1 protein (RIG-1) is a cytoplasmic DExD/H box RNA helicase belonging to the helicase family and containing 2 CARD domains within the N-terminus, a repressor domain (RD), a helicase ATP-binding domain and a helicase C-terminal binding domain. RIG-1 is able to bind the 5’-triphosphate RNA of single or double-stranded viral RNA. After recognizing and binding viral RNA, RIG-1 protein initializes type I IFN production, and within fibroblasts and dendritic cells plays a central role in the regulation of type I IFN production in response to viral presence. There are four known isoforms resulting from alternative splice variants. View All» Species Reactivity: Human View All» Species Reactivity Note: Demonstrated to react with Human. Other homologies: Rhesus Monkey (99% sequence homology). View All» Application Notes: Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Paz, S., et al. (2011). Cell Research. 1-16.) View All» Control: SeV treated and untreated A549 cell lysates View All» Quality Assurance: Evaluated by Western Blot in SeV treated and untreated A549 cell lysates.Western Blot Analysis: 0.05 µg/mL of this antibody detected RIG-I on 10 µg of SeV untreated and treated A549 cell lysates. View All» Purification Method: Affinity purified View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: O95786 View All» Entrez Gene Number: NP_055129.2 View All» Gene Symbol: DDX58 View All» Alternate Names:

• Probable ATP-dependent RNA helicase DDX58

• DEAD box protein 58

• Retinoic acid-inducible gene 1 protein

• RIG-1

• Retinoic acid-inducible gene I protein

• RIG-I
  View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
  • Western Blotting

  • Immunoprecipitation
    View All» Entrez Gene Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). Essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV (By similarity). CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate.COFACTOR: Zinc.SUBUNIT STRUCTURE: Monomer; maintained as a monomer in an autoinhibited state. Upon viral dsRNA binding and conformation shift, homomultimerizes and interacts with MAVS. Interacts with DHX58/LGP2, IKBKE, TBK1 and TMEM173/STING. Interacts (via CARD domain) with TRIM25 (via SPRY domain). Interacts with RNF135. Interacts with CYLD. Interacts with NLRC5; blocks the interaction of MAVS to DDX58.SUBCELLULAR LOCATION: Cytoplasm. Note: Colocalized with TRIM25 at cytoplasmic perinuclear bodies. TISSUE SPECIFICTY: Present in vascular smooth cells (at protein level). INDUCTION: By bacterial lipopolysaccharides (LPS) in endothelial cells. By IFN-alphas, IFNB1/IFN-beta and IFNG/IFN-gamma. DOMAIN: The repressor domain controls homomultimerization and interaction with MAVS Probable. The helicase domain is responsible for dsRNA recognition. The 2 CARD domains are responsible for interaction with and signaling through MAVS.The second CARD domain is the primary site for 'Lys-63'-linked ubiquitination. PTM: Isgylated. Conjugated to ubiquitin-like protein ISG15 upon IFN-beta stimulation.Ubiquitinated. Undergoes 'Lys-63'-linked ubiquitination. Lys-172 is the critical site for TRIM25-mediated ubiquitination, for MAVS binding and to induce anti-viral signal transduction. Lys-154, Lys-164 and Lys-172 are critical sites for RNF135-mediated ubiquitination. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains. SEQUENCE SIMILARITIES: Belongs to the helicase family.Contains 2 CARD domains.Contains 1 helicase ATP-binding domain.Contains 1 helicase C-terminal domain. View All» Product Name: Anti-RIG-I (N-Terminus) View All» Concentration: 0.5 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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