货号：06-1295

品牌：Merck Millipore

规格：100UL

目录价：￥5053.00

市场价格：￥4295.05

会员价格：￥4042.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-CDT1 Antibody | 06-1295 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes CDT1. View All» Molecular Weight: ~60 kDa View All» Immunogen: KLH-conjugated linear peptide corresponding to human CDT1. View All» Background Information: CDT1 (Chromatin Licensing and DNA replication Factor 1) is an essential component of the cell cycle machinery. It plays an important role in insuring that there is only one round of DNA replication per cell cycle. CDT1 is a known substrate of Cdk2 and Cdk4 (Liu, E. 2004). It is presented in G1 of the cell cycle and is degraded as cells enter S phase (Liu, E. 2004). In higher eukaryotes, CDT1 is known to interact with Geminin, a protein that is degraded during mitosis, that inhibits it. CDT1 is marked for degradation by two E3 ligases: SCF complex and the CUL4-DDB1 complex (Sansam, 2006). View All» Species Reactivity:

• Human

• Rat

• Mouse
  View All» Species Reactivity Note: Proven to react with human, mouse and rat. View All» Application Notes: Western Blot Analysis: A 1:1,000 dilution from a previous lot detected CDT1 in C2C12, Hek293, HeLa, HepG2, Huvec, Jurkat, L6, mouse brain, NIH/3T3, PC3, and RAW264.7 cell lysates. View All» Control: HeLa cell lysate View All» Quality Assurance: Evaluated by Western Blot in HeLa cell lysate.Western Blot Analysis: A 1:1,000 dilution of this antibody detected CDT1 in 10 µg of HeLa cell lysate. View All» Purification Method: Unpurified View All» Presentation: Rabbit polyclonal serum with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: Q9H211 View All» Entrez Gene Number: NP_005905.1 View All» Gene Symbol:
  • DUP

  • RIS2
    View All» Alternate Names:
    • DNA replication factor

    • Double parked homolog

    • Double parked, Drosophila, homolog of

    • chromatin licensing and DNA replication factor 1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» UniProt Summary: FUNCTION: Cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. Binds DNA in a sequence-, strand-, and conformation-independent manner. Potential oncogene. SUBUNIT STRUCTURE: Interaction with GMNN inhibits both binding of the MCM complex to origins of replication and DNA-binding activity. SUBCELLULAR LOCATION: Nucleus. DEVELOPMENTAL STAGE: Present during G1 and early S phase of the cell cycle. Degraded during the late S, G2, and M phases. PTM : Ubiquitinated, leading to its subsequent degradation. The interaction with GMNN protects it against ubiquitination. Phosphorylated by cyclin A-dependent kinases which results in the binding of CDT1 to the F-box protein SKP2 and subsequent degradation. Binding to GMNN is not affected by phosphorylation.SEQUENCE SIMILARITIES: Belongs to the Cdt1 family.SEQUENCE CAUTION: The sequence AF070552 differs from that shown. Reason: Frameshift at positions 278 and 312. View All» Product Name: Anti-CDT1 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Serum View All» Host: Rabbit View All»
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