货号：06-1328

品牌：Merck Millipore

规格：

目录价：￥3724.00

市场价格：￥3165.40

会员价格：￥2979.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Themis Antibody | 06-1328 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes Themis at the C-terminus. View All» Molecular Weight: ~72 kDa observed. UniProtKB/Swiss-Prot Q8BGW0 (THMS1_MOUSE) entry describes 5 isoforms produced by alternative splicing: Isoform 1 at 72.780 kDa, Isoform 2 at 69.544 kDa, Isoform 3 at 68.293 kDa, Isoform 4 at 59.082 kDa, and Isoform 5 at 30.040 kDa. View All» Epitope: C-terminus View All» Immunogen: KLH-conjugated linear peptide corresponding to the C-terminus of mouse Themis. View All» Background Information: Thymocyte-expressed molecule involved in selection (Themis) is a highly conserved protein that has been shown to serve a key role in regulating T-cell development through TCR signaling, especially through the moderation of phosphorylation and calcium influx. It is also a critical component in the later stages of thymocyte development through the regulation of positive/negative T-cell selection, and is required for integration and maintenance of signals necessary to T-cell lineage commitment and maturation. Themis expression is observed in thymus, spleen, thymocytes between Pre-TCR and checkpoints positive in selectin, and at low levels in mature T-cells. View All» Species Reactivity: Mouse View All» Species Reactivity Note: Demonstrated to react with mouse. Rat (73% sequence homology). View All» Application Notes: Western Blot (Snap i.d.) Analysis: 2 µg/mL from a previous lot detected Themis in 10 µg of mouse thymocyte tissue lysate.Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC. (Vasily Rybakin, PhD., Gascoigne lab, Scripps Research Institute, La Jolla, CA) View All» Control: Mouse thymocyte tissue lysate View All» Quality Assurance: Evaluated by Western Blot in mouse thymocyte tissue lysate.Western Blot Analysis: 0.1 µg/mL of this antibody detected Themis in 10 µg of mouse thymocyte tissue lysate. View All» Purification Method: Affinity Purified View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: Q8BGW0 View All» Entrez Gene Number: NP_848781 View All» Gene Symbol:

• Themis

• TSEPA
  View All» Alternate Names:
  • thymocyte selection associated

  • thymocyte selection pathway associated

  • chromosome 6 open reading frame 190

  • chromosome 6 open reading frame 207

  • protein THEMIS

  • Thymocyte-expressed molecule involved in selection
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Flow Cytometry
      View All» UniProt Summary: FUNCTION: Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk. SUBUNIT STRUCTURE: Interacts with PLCG1, ITK and GRB2. SUBCELLULAR LOCATION: Cytoplasm. Nucleus TISSUE SPECIFICTY: Expressed in the thymus and to a lesser extent in the spleen but not detectable in nonlymphoid tissues. Highly expressed in thymocytes between the pre-T-cell antigen receptor (pre-TCR) and positive-selection checkpoints and expressed at low level in mature T-cells (at protein level). INDUCTION: Down-regulated by stimulation through the alpha-beta TCR. PTM: Phosphorylated on Tyr residues quickly after TCR stimulation. DISRUPTION: phenotype Mice are viable, are born at the expected mendelian frequency and present no gross abnormalities. They however display a blockade in late T-cell development with defects in thymocyte selection. The number of transitional CD4+CD8(int) thymocytes as well as CD4+ or CD8+ single-positive thymocytes is lower. Thymocytes show defective positive selection, resulting in fewer mature thymocytes. Negative selection is also impaired. A greater percentage of T-cells have CD4+CD25+Foxp3+ regulatory and CD62L(lo)CD44(hi) memory phenotypes compared to wild-type T-cells. SEQUENCE SIMILARITIES: Belongs to the themis family. View All» Product Name: Anti-Themis View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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