货号：06-496

品牌：Merck Millipore

规格：

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-PI3 Kinase p85 Antibody, N-SH2 domain | 06-496 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Recognizesthe p85 alpha subunit of PI3 Kinase (all isoforms). View All» Molecular Weight: 85 kDa View All» Epitope: N-SH2 View All» Immunogen: GST fusion protein containing the N-SH2 region of rat PI3 Kinase. View All» Isotype: IgG View All» Background Information: The enzyme phosphatidylinositol 3 kinase (PI3 kinase) is a lipid kinase that is thought to phosphorylate second messengers in growth signalling pathways. PI3K exists as a heterodimer of catalytic 110 kDa (p110) and regulatory 85 kDa (p85, p50 or p55) subunits. The p85 unit consists of two closely related proteins: p85 α, which binds tightly to the catalytic subunit and p85 β, a protein of unknown function. They share 62% amino acid sequence identity. p85 α lacks PI3 kinase activity, but may serve as regulator of the catalytic subunit, p110, by acting as the link between PI3K and the ligand activated receptor. Both isoforms bind to activated receptors and serve as tyrosine kinase substrates. View All» Species Reactivity:

• Human

• Rat

• Mouse

• Monkey
  View All» Species Reactivity Note: Human, Mouse, Monkey, Rat. Reactivity with other species has not been confirmed. View All» Application Notes: Immunoprecipitation: 4 µg of a previous lot immuno-precipitated the p85 subunit of PI3 Kinase as a complex with the p110 kDa subunit from 500 µg of Jurkat RIPA lysate. View All» Control: RIPA lysates from human Jurkat T cells. View All» Quality Assurance: Routinely evaluated by western blot on RIPA lysates from human Jurkat T cells.Western Blot Analysis: 0.5-2 µg/mL of this lot detected PI3 Kinase in RIPA lysates from human Jurkat T cells. View All» Purification Method: Protein A chromatography View All» Presentation: Purified rabbit polyclonal IgG in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide. Frozen solution. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of the product, centrifuge the original vial after thawing and prior to removing the cap.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: P27986 View All» Entrez Gene Number: NP_852556 View All» Gene Symbol:
  • PIK3R1

  • PI3K

  • p85-ALPHA

  • GRB1
    View All» Alternate Names:
    • PI3-kinase p85 subunit alpha

    • PtdIns-3-kinase p85-alpha

    • phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85alpha)

    • phosphatidylinositol 3-kinase, regulatory, 1

    • phosphatidylinositol 3-kinase-associated p-85 alpha

    • phosphoinositide-3-kinase, regulatory subunit 1 (alpha)

    • phosphoinositide-3-kinase, regulatory subunit 1 (p85 alpha)

    • phosphoinositide-3-kinase, regulatory subunit, polypeptide 1 (p85alpha)
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunoprecipitation

      • Western Blotting
        View All» Entrez Gene Summary: Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in three transcript variants encoding different isoforms. View All» UniProt Summary: FUNCTION: SwissProt: P27986 # Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues.SIZE: 724 amino acids; 83598 Da SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunits. Interacts with phosphorylated TOM1L1. Interacts with phosphorylated LIME1 upon TCR and/or BCR activation. Interacts with SOCS7. Interacts with RUFY3 (By similarity). Interacts with phosphorylated LAT, LAX1 and TRAT1 upon TCR activation. Interacts with CBLB. Interacts with HIV-1 Nef to activate the Nef associated p21-activated kinase (PAK). This interaction depends on the C- terminus of both proteins and leads to increased production of HIV. Interacts with HCV NS5A. The SH2 domains interact with the YTHM motif of phosphorylated INSR in vitro. Also interacts with tyrosine-phosphorylated IGF1R in vitro. Interacts with CD28 and CD3Z upon T-cell activation. Interacts with IRS1.TISSUE SPECIFICITY: Isoform 2 is expressed in skeletal muscle and brain, and at lower levels in kidney and cardiac muscle. Isoform 2 and isoform 4 are present in skeletal muscle (at protein level).DOMAIN: SwissProt: P27986 The SH3 domain mediates the binding to CBLB, and to HIV-1 Nef.PTM: Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.DISEASE:SwissProt: P27986 # Defects in PIK3R1 are a cause of severe insulin resistance.SIMILARITY: Belongs to the PI3K p85 subunit family. & Contains 1 Rho-GAP domain. & Contains 2 SH2 domains. & Contains 1 SH3 domain. View All» Brand Family: Upstate View All» Product Name: Anti-PI3 Kinase p85 Antibody, N-SH2 domain | 06-496 View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 250 µg View All» Format: Purified View All» Host: Rabbit View All»
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