货号：06-570

品牌：Merck Millipore

规格：200Ug

目录价：￥7049.00

市场价格：￥5991.65

会员价格：￥5639.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-Histone H3 (Ser10) Antibody, Mitosis Marker | 06-570 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: This antibody recognizes Histone H3 when phosphorylated at Ser10. View All» Molecular Weight: 17 kDa View All» Epitope: Ser10 View All» Immunogen: Linear peptide corresponding to human Histone H3 at Ser10. View All» Modifications: Phosphorylation View All» Background Information: Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. View All» Species Reactivity:

• Mouse

• Human
  View All» Species Reactivity Note: Demonstrated to react with human and mouse. View All» Application Notes: Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Histone H3 in HeLa and A431 cells.Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated phospho Histone H3 (Ser10) from HeLa acid extract and Colcemid treated HeLa acid extracted RIPA lysate.Beadlyte® Assay Analysis: A representative lot recognizes Histone H3 phosphorylated on Ser10 by Luminex assay. View All» Control: Colcemid treated HeLa acid extract View All» Quality Assurance: Evaluated by Western Blot in Colcemid treated HeLa acid extract.Western Blot Analysis: 0.5 – 1 µg/ml of this antibody detected Histone H3 on 10 µg of Colcemid treated HeLa acid extract. View All» Purification Method: Protein A purfied View All» Presentation: Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.150 mM NaCl and 0.05% sodium azide. View All» Storage Conditions: 2-8°C for up to 6 months. View All» UniProt Number: Q16695 View All» Entrez Gene Number: NP_003484.1 View All» Gene Symbol:
  • H3.3A

  • H3.3B

  • H3F3

  • H3F3A
    View All» Alternate Names:
    • H3S10P

    • Histone H3 (phospho S10)

    • H3 histone, family 3A

    • H3 histone, family 3B

    • H3 histone, family 3B (H3.3B)
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunocytochemistry

      • Immunoprecipitation

      • Western Blotting
        View All» Entrez Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene contains introns and its mRNA is polyadenylated, unlike most histone genes. The protein encoded is a replication-independent member of the histone H3 family. View All» UniProt Summary: FUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.SUBUNIT STRUCTURE: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Expressed in testicular cells.DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 By similarity.Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription By similarity.Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression By similarity.Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin By similarity.Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation By similarity.Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin.Ubiquitinated By similarity.SEQUENCE SIMILIARITIES: Belongs to the histone H3 family. View All» Brand Family: Upstate View All» Product Name: Anti-phospho-Histone H3 (Ser10) Antibody, Mitosis Marker | 06-570 View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 200 µg View All» Format: Purified View All» Host: Rabbit View All»
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