货号：06-574

品牌：Merck Millipore

规格：200Ug

目录价：￥6895.00

市场价格：￥5860.75

会员价格：￥5516.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-TrkA Antibody | 06-574 View All» Replaces: CBL770 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Recognizes TrkA, Mr 140 kDa. Does not cross-react with TrkB or TrkC. View All» Molecular Weight: 140 kDa View All» Immunogen: Rabbits immunized with bacterially expressed purified protein corresponding to the entire extracellular domain of rat TrkA receptor. View All» Isotype: IgG View All» Background Information: TrkA, apparent molecular weight 140 kDa, is a high affinity nerve growth factor (NGF). The Trk proto-oncogene family contains four members, TrkA, TrkB, TrkC, and TrkE, which are variably expressed throughout the central and peripheral nervous systems. TrkA binds to nerve growth factor (NGF) and autophosphorylates on tyrosine residues (Tyr490, Tyr674, Tyr675, Tyr751 and Tyr785) to activate multiple downstream effector proteins. Phosphorylation at Tyr490 is required for Shc association and subsequent activation of the Ras-MAP kinase-signaling cascade, which leads to activation of Elk-1-dependent gene transcription and neurite growth. Phosphorylations at Tyr674 and Tyr675 lie within the catalytic domain of TrkA tyrosine kinase and reflect Trk kinase activity. Additionally, phosphorylation at Tyr751 is required for PI3-kinase association and activation of the Akt signaling cascade. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Rat, mouse and human. Reactivity with other species has not been confirmed. View All» Application Notes: Immunoprecipitation: 4 μg of a previous lot immunoprecipitated TrkA from 500 μg of PC-12 RIPA lysate.Immunocytochemistry: 10 μg/mL of a previous lot of this antibody showed positive immunostaining for TrkA in PC-12 cells fixed with 4% paraformaldehyde. View All» Control: EGF-stimulated A431 cel lysate, rat brain tissue. Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. View All» Quality Assurance: Routinely evaluated by Western Blot on RIPA lysates from human A431 cells, mouse 3T3 or rat PC-12 cell lysates.Western Blot Analysis: 0.5-2 μg/mL of this lot detected TrkA in RIPA lysates from human A431 cells; a previous lot detected TrkA in mouse 3T3 and rat PC-12 cell lysates. View All» Purification Method: Protein A purfied View All» Presentation: Purified rabbit IgG in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, 0.1% sodium azide. Frozen at -20°C. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage. View All» UniProt Number: P04629 View All» Entrez Gene Number: NM_021589 View All» Gene Symbol:
  • DKFZp781I14186

  • EC 2.7.10.1

  • MTC

  • OTTHUMP00000038736

  • TRK

  • TRK1

  • TRKA

  • Trk-A

  • p140-TrkA
    View All» Alternate Names:
    • Neurotrophic tyrosine kinase receptor type

    • Oncogene TRK

    • TRK1-transforming tyrosine kinase protein

    • high affinity nerve growth factor receptor

    • neurotrophic tyrosine kinase, receptor, type 1

    • tyrosine kinase receptor A
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunocytochemistry

      • Immunoprecipitation

      • Western Blotting
        View All» Entrez Gene Summary: This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date. View All» UniProt Summary: FUNCTION: SwissProt: P04629 # Required for high-affinity binding to nerve growth factor (NGF), neurotrophin-3 and neurotrophin-4/5 but not brain- derived neurotrophic factor (BDNF). Known substrates for the Trk receptors are SHC1, PI 3-kinase, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system as well as establishment of thermal regulation via sweating. Activates ERK1 by either SHC1- or PLC-gamma-1-dependent signaling pathway.SIZE: 796 amino acids; 87497 Da SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. Binds SH2B2. Interacts with SQSTM1 which bridges NTRK1 to NGFR (By similarity).SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein (By similarity). Note=Endocytosed to the endosomes upon treatment of cells with NGF (By similarity).TISSUE SPECIFICITY: Isoform TrkA-II is primarily expressed in neuronal cells; isoform TrkA-I is found in non-neuronal tissues.PTM: Ligand-mediated auto-phosphorylation. Interaction with SQSTM1 is phosphotyrosine-dependent.DISEASE: SwissProt: P04629 # Defects in NTRK1 are a cause of congenital insensitivity to pain with anhidrosis (CIPA) [MIM:256800]. CIPA is characterized by a congenital insensitivity to pain, anhidrosis (absence of sweating), absence of reaction to noxious stimuli, self-mutilating behavior, and mental retardation. This rare autosomal recessive disorder is also known as congenital sensory neuropathy with anhidrosis or hereditary sensory and autonomic neuropathy type IV or familial dysautonomia type II. & Chromosomal aberrations involving NTRK1 are a cause of thyroid papillary carcinoma (PACT) [MIM:188550]. Translocation t(1;3)(q21;q11) with TFG generates the TRKT3 (TRK-T3) transcript by fusing TFG to the 3'-end of NTRK1; a rearrangement with TPM3 generates the TRK transcript by fusing TPM3 to the 3'-end of NTRK1. & Chromosomal aberrations involving NTRK1 are a cause of thyroid papillary carcinoma (PACT) [MIM:188550]. Intrachromosomal rearrangement that links the protein kinase domain of NTRK1 to the 5'-end of the TPR gene forms the fusion protein TRK-T1. TRK-T1 is a 55 kDa protein reacting with antibodies against the C-terminus of the NTRK1 protein.SIMILARITY: SwissProt: P04629 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. & Contains 2 Ig-like C2-type (immunoglobulin-like) domains. & Contains 3 LRR (leucine-rich) repeats. & Contains 1 protein kinase domain. ... hide » see more » View All» Brand Family: Upstate View All» Product Name: Anti-TrkA Antibody | 06-574 View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 200 µg View All» Target / Localization: Plasma membrane View All» Format: Purified View All» Host: Rabbit View All»
        © 金山科研平台是专业的授权总代理区域代理经销平台。
        © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
        © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
        © 本文地址：https://www.16ao.com/thread-134425.htm
        © Merck Millipore代理06-574 Anti-TrkA Antibody;抗-TrkA抗体store at -20℃，产品报价联系微信jinshanbio