货号：06-579

品牌：Merck Millipore

规格：

目录价：￥3908.00

市场价格：￥3321.80

会员价格：￥3126.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Gab1 Antibody, CT | 06-579 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Specific for Gab1. No other known protein shares more than 10 amino acids with the immunizing sequence. View All» Molecular Weight: 115 kDa View All» Epitope: C-terminus View All» Immunogen: 31 residue peptide sequence corresponding to C-terminal residues 664-694 of Gab1, (CQKTLALKSTREAWTDGRQSTESETPAKSVK). View All» Isotype: IgG View All» Background Information: Gab1 is a 115 kDa multiple docking protein that plays an essential role in cellular growth, transformation and apoptosis. Gab1 can be phosphorylated by multiple receptor tyrosine kinase (RTKs), including: insulin receptor (IR), platelet derived growth factor receptor beta] (PDGFR-β]), hepatocyte growth factor/scatter factor receptor (HGFR/SFR or c Met), and epidermal growth factor receptor (EGF), as well as in response to cell cell adhesion. Gab1 is tyrosine phosphorylated on at least 16 sites, some of which serve as binding sites for phosphoatidylinositol 3 kinase (PI3K), Grb2, PLC gamma 1, Nck, and SHP2. Phosphorylation of Gab1 on tyrosines 627 and 659 is critical for its binding to SHP2, and for activation of the ERK/MAPK pathway in response to EGF. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Human, mouse and rat. Reactivity with other species has not been confirmed. View All» Application Notes: Immunoprecipitation: 4 μg of this lot immunoprecipitated Gab1 from 500 μg of a human A431 carcinoma cell RIPA lysate.Immunocytochemistry: 10 μg/mL of a previous lot showed positive immunostaining for Gab1 in A431 cells fixed with 4% paraformaldehyde. View All» Control: NIH/3T3 cytoplasmic lysate. Included Positive Antigen Control: Catalog # 12-301 non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels. View All» Quality Assurance: Routinely evaluated by Western Blot on NIH/3T3 cytoplasmic lysate.Western Blot Analysis: 0.5-2 μg/mL of this lot detected Gab1 in RIPA lysates from human A431 carcinoma cells. View All» Purification Method: Protein A purfied View All» Presentation: Purified IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide. Frozen solution. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: Q13480 View All» Entrez Gene Number: NM_207123 View All» Gene Symbol: GAB1 View All» Alternate Names:
  • GRB2-associated binder

  • GRB2-associated binding protein

  • Growth factor receptor bound protein 2-associated protein
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Immunocytochemistry

    • Immunohistochemistry (Paraffin)

    • Immunoprecipitation

    • Western Blotting
      View All» Entrez Gene Summary: The protein encoded by this gene is a member of the IRS1-like multisubstrate docking protein family. The encoded protein is an important mediator of branching tubulogenesis and plays a central role in cellular growth response, transformation and apoptosis. Two transcript variants encoding different isoforms have been found for this gene. View All» UniProt Summary: FUNCTION: SwissProt: Q13480 # Probably involved in EGF and insulin receptor signaling.SIZE: 694 amino acids; 76616 Da SUBUNIT: Interacts with GRB2 and with other SH2-containing proteins. Interacts with phosphorylated LAT2.PTM: Phosphorylated on tyrosine residue(s) by the epidermal growth factor receptor (EGFR) and the insulin receptor (INSR). Tyrosine phosphorylation of GAB1 mediates interaction with several proteins that contain SH2 domains.SIMILARITY: SwissProt: Q13480 ## Belongs to the GAB family. & Contains 1 PH domain. View All» Brand Family: Upstate View All» Product Name: Anti-Gab1 Antibody, CT | 06-579 View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 200 µg View All» Format: Purified View All» Host: Rabbit View All»
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