货号：06-747

品牌：Merck Millipore

规格：200Ug

目录价：￥5985.00

市场价格：￥5087.25

会员价格：￥4788.00

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Neomycin Phosphotransferase II Antibody | 06-747 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Recognizes NPT II, Mr ~30 kDa View All» Molecular Weight: ~30 kDa View All» Immunogen: Full length neomycin phosphotransferase (NPT) II View All» Modifications: Phosphorylation View All» Isotype: IgG View All» Background Information: Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin®, G418 Sulfate) in mammalian cells. These antibiotics bind to ribosomal components and inhibit protein synthesis. NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell. Immunoblot analysis of transfected cell lysates with anti NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids. View All» Species Reactivity: Bacteria View All» Species Reactivity Note: Human. Expected to react with mouse and rat based on sequence homology. View All» Application Notes: Western Blot Analysis: 1-2 μg/mL dilutions of a previous lot detected NPT II in RIPA lysates from Cos-1 cells transfected with pUSE, harboring the neomycin resistance gene. View All» Control: RIPA lysates from Cos-1 cells transfected with pUSE, harboring the neomycin resistance gene View All» Quality Assurance: Routinely evaluated by Western Blot on transiently transfected Cos-1 cells.Western Blot Analysis:1-2 μg/mL of a previous lot detected NPT II in RIPA lysate from Cos-1 cells transfected with pUSE, harboring the neomycin resistance gene. View All» Purification Method: Protein A purfied View All» Presentation: Purified rabbit polyclonal IgG in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide before the addition of glycerol to 30%. Liquid at -20°C. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt.Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P00552 View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Brand Family: Upstate View All» Product Name: Anti-Neomycin Phosphotransferase II Antibody | 06-747 View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 200 µg View All» Format: Purified View All» Host: Rabbit View All»