货号：06-866

品牌：Merck Millipore

规格：200UL

目录价：￥7049.00

市场价格：￥5991.65

会员价格：￥5639.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-acetyl-Histone H4 Antibody | 06-866 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Acetylated histone H4, acetylated histone H2B from Tetrahymena and weakly cross-reacts with acetylated histone H2B from HeLa cells, may cross-react with other acetylated proteins. View All» Molecular Weight: 10 kDa View All» Epitope: a.a. 2-19 View All» Immunogen: KLH-conjugated peptide corresponding to a.a. 2-19 of Tetrahymena histone H4 [AGGAcKGGAcKGMGAcKVGAAcKRHS-C], acetylated on lysines 5, 8, 12 and 16. View All» Modifications: Acetylation View All» Isotype: IgG View All» Background Information: Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). View All» Species Reactivity:

• Human

• Tetrahymena

• Eukaryote
  View All» Species Reactivity Note: Human and Tetrahymena. Other species not tested, but expected to cross-react since Histone H4 is well conserved. View All» Application Notes: Chromatin Immunoprecipitation: 5-10 μL of this lot immunoprecipitated transcriptionally active chromatin containing acetylated histone H4 from 2 X 106 serum stimulated HeLa cells. View All» Control: TSA-treated Jurkat cells. For a negative control, perform a no-antibody immunoprecipitation by incubating the supernatant fraction with 60μl of Salmon Sperm DNA/Protein A Agarose- 50% Slurry. Transcriptionally unactivated DNA samples should be prepared as controls for PCR. View All» Quality Assurance: Routinely evaluated by western blot on Tetrahymena macronuclei or acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate.Western Blot Analysis: 1:2000 dilution of this lot detected acetylated histone H4 in acid extracted proteins from HeLa cells treated with 5 mM sodium butyrate. Sodium butyrate, an inhibitor of deacetylases, was used to enhance detection of acetylated histone H4. View All» Purification Method: Unpurified View All» Presentation: Whole antiserum containing 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt.Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. View All» UniProt Number: P62805 View All» Entrez Gene Number: NM_175054 View All» Gene Symbol:
  • HIST1H4A

  • H4/J

  • HIST1H4F

  • HIST1H4L

  • H4FN

  • H4FA

  • H4FH

  • HIST1H4H

  • H4F2

  • H4FM

  • H4/A

  • H4FK

  • H4FG

  • HIST2H4

  • H4FB

  • H4/K

  • HIST1H4I

  • H4/N

  • HIST1H4B

  • H4FE

  • H4/M

  • H4/a

  • IST1H4E

  • HIST4H4

  • HIST2H4A

  • H4FC

  • H4FI

  • H4/H

  • HIST1H4C

  • H4/G

  • HIST1H4K

  • 4FJ

  • H4FD

  • H4/I

  • H4/B

  • H4/D

  • H4/C

  • HIST1H4J

  • HIST1H4D

  • H4/E
    View All» Alternate Names:
    • H4 histone family, member A

    • histone 1, H4a

    • histone cluster 1, H4a
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunocytochemistry

      • Chromatin Immunoprecipitation (ChIP)
        View All» Entrez Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. View All» UniProt Summary: FUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.SIZE: 103 amino acids; 11367 Da SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.SUBCELLULAR LOCATION: Nucleus.PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family. View All» Brand Family: Upstate View All» Product Name: Anti-acetyl-Histone H4 Antibody | 06-866 View All» Concentration: Variable View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 200 µL View All» Format: Serum View All» Host: Rabbit View All»
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