货号：07-1024

品牌：Merck Millipore

规格：EA

目录价：￥4008.00

市场价格：￥3406.80

会员价格：￥3206.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-EGFR Antibody, cytoplasmic domain | 07-1024 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: Detects EGFR View All» Molecular Weight: 180 kDa View All» Epitope: Cytoplasmic Domain View All» Immunogen: GST fusion protein corresponding to the cytoplasmic domain of human EGFR. View All» Background Information: EGFR (Epidermal Growth Factor Receptor; ErbB-1; HER1) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The EGF receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer. EGFR dimerization stimulates its intrinsic intracellular protein-tyrosine kinase activity. As a result, autophosphorylation of five tyrosine (Y) residues (Y992, Y1045, Y1068, Y1148 and Y1173) in the C-terminal domain of EGFR occurs. This autophosphorylation elicits downstream activation and signaling by several other proteins that associate with the phosphorylated tyrosines through their own phosphotyrosine-binding SH2 domains. These downstream signaling proteins initiate several signal transduction cascades, principally the MAPK, Akt and JNK pathways, leading to DNA synthesis and cell proliferation. View All» Species Reactivity: Human View All» Species Reactivity Note: Human. Other species have not been tested. View All» Quality Assurance: Routinely evaluated by Western Blot.Western Blot Analysis: This lot detected EGFR at 1:2,000 dilution in A431 cell lysate resolved via SDS-PAGE and transferred to PVDF. View All» Purification Method: Immunogen Affinity Purified View All» Presentation: Affinity purified rabbit polyclonal in 0.1M Tris-Glycine (pH 7.4) with 150mM NaCl and 0.05% NaN3. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt.Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. View All» UniProt Number: P00533 View All» Entrez Gene Number: NP_005219.2 View All» Gene Symbol:

• EC 2.7.10.1

• ERBB

• ERBB1

• HER1

• PIG61

• mENA
  View All» Alternate Names:
  • Receptor tyrosine-protein kinase ErbB-1

  • avian erythroblastic leukemia viral (v-erb-b) oncogene homolog

  • cell growth inhibiting protein 40

  • cell proliferation-inducing protein 61

  • epidermal growth factor receptor

  • epidermal growth factor receptor (avian erythroblastic leukemia viral(v-erb-b) oncogene homolog

  • epidermal growth factor receptor (erythroblastic leukemia viral(v-erb-b) oncogene homolog, avian)
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq] View All» UniProt Summary: Function: Receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin. Ref.31Isoform: 2/truncated isoform may act as an antagonist. Ref.31Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.Subunit structure: Binds RIPK1. CBL interacts with the autophosphorylated C-terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1. Binds MUC1. Ref.31 Ref.25 Ref.29 Ref.30 Ref.35Subcellular location: Cell membrane; Single-pass type I membrane protein. Isoform: 2: Secreted. Tissue specificity: Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers. Ref.38Post-translational modification: Phosphorylation of Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Ref.16 Ref.24 Ref.28 Ref.32 Ref.33 Ref.34 Ref.36 Ref.37 Ref.39 Ref.40 Ref.41 Ref.42Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.Involvement in disease: Defects in EGFR are associated with lung cancer [MIM:211980].Miscellaneous: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.Sequence similarities: Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.Contains 1 protein kinase domain. View All» Product Name: Anti-EGFR, cytoplasmic domain View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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