货号：07-1232

品牌：Merck Millipore

规格：200UL

目录价：￥5677.00

市场价格：￥4825.45

会员价格：￥4541.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Notch 1 Antibody, N-Terminus | 07-1232 View All» Replaces: 07-1232 is a recommended replacement for AB5709 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: Notch 1, cleaved N terminal. Only the cleaved intracellular (activated) form is detected. No reactivity is detected against mouse N2, N3 or N4. This epitope is exposed only after gamma secretase cleavage and is not accessible in the uncleaved form. View All» Molecular Weight: ~80 kDa View All» Epitope: Cleaved N-Terminus View All» Immunogen: Synthetic peptide from the N-terminal sequence of the cleaved N intracellular domain (NICD) human Notch 1. View All» Isotype: IgG View All» Background Information: Notch is a family of single-pass transmembrane receptor proteins that are synthesized in the endoplasmic reticulum as an inactive form and are proteolytically cleaved on an extracellular site by a furin-like convertase (S1 cleavage) in the trans-golgi network after the recognition of the RQRR sequence before it reaches the plasma membrane as heterodimers to yield an active, ligand-accessible form. The Notch family is comprised of 4 members (1-4) whose ligands include the Delta and Jagged family of ligands. These ligands cause proteolysis of Notch to liberate the intracellular domain. Cleavage results in a C-terminal fragment N(TN) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase between gly1743 and val1744 (S3 cleavage) to release the intracellular domain (NICD) from the membrane. That domain translocates to the nucleus and initiates transcription events by binding the DNA binding protein CSL. The notch family members are involved cell differentiation and development. View All» Species Reactivity:

• Human

• Mouse
  View All» Species Reactivity Note: Human and mouse. The antibody is also expected to react with rat due to immunogen sequence homology. Other species have not been tested. View All» Application Notes: ELISA: A previous lot of this antibody was diluted to 1:20,000-1:60,000 and used in a standard sandwich ELISA assay against the peptide immunogen.Immunohistochemistry(paraffin):Representative testing from a previous lot.Optimal Staining of Notch-1 With Citrate pH 6.0 Epitope Retrieval: Uterine CancerELISA: A previous lot of this antibody was diluted to 1:20,000-1:60,000 and used in a standard sandwich ELISA assay against the peptide immunogen. View All» Control: Jurkate Cell Lysates. In fetal tissues, use spleen, brain stem and lung. In adult tissues use mainly lymphoid tissues. View All» Quality Assurance: Routinely evaluated by Western Blot on Jurkat lysates.Western Blot Analysis: 1:500 dilution of this lot detected cleaved NOTCH1 on 10 μg of Jurkat lysates. View All» Presentation: Rabbit polyclonal IgG serum in buffer containing 0.02 M Potassium Phosphate, 0.15 M NaCl, pH 7.2, containing 0.1% sodium azide. View All» Storage Conditions: Stable for 6 months at -20°C in undiluted aliquots from date of receipt. Avoid repeated freeze/thaw cycles.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: P46531 View All» Entrez Gene Number: NM_017617.3 View All» Gene Symbol:
  • NOTCH1

  • notch1

  • hN1

  • TAN1
    View All» Alternate Names:
    • Neurogenic locus notch homolog protein 1 precursor

    • Notch (Drosophila) homolog 1 (translocation-associated)

    • Notch 1

    • Notch homolog 1, translocation-associated (Drosophila)

    • Translocation-associated notch protein TAN-1

    • Neurogenic locus notch homolog protein 1
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • ELISA

      • Western Blotting

      • Immunohistochemistry (Paraffin)
        View All» Entrez Gene Summary: This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play multiple roles during development. View All» UniProt Summary: FUNCTION: SwissProt: P46531 # Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia (By similarity).SIZE: 2556 amino acids; 272500 Da View All» Product Name: Anti-Notch 1 Antibody, N-Terminus | 07-1232 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 200 μL View All» Format: Serum View All» Host: Rabbit View All»
        © 金山科研平台是专业的授权总代理区域代理经销平台。
        © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
        © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
        © 本文地址：https://www.16ao.com/thread-134609.htm
        © Merck Millipore代理07-1232 Anti-Notch 1 Antibody, NT;store at -20℃，产品报价联系微信jinshanbio