货号：07-1413

品牌：Merck Millipore

规格：50Ug

目录价：￥5207.00

市场价格：￥4425.95

会员价格：￥4165.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-GSK-3b Antibody, a.a. 335-349 | 07-1413 View All» Replaces: 07-1413 is a recommended replacement for AB8687 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody detects a strong 47 kDa protein, corresponding to the expected molecular mass of GSK3 beta. View All» Molecular Weight: This antibody detects a strong 47 kDa protein. View All» Epitope: a.a. 335-349 View All» Immunogen: Rat GSK3 beta synthetic peptide (aa. 335-349), conjugated to KLH. This sequence is 100% identical to human GSK3 beta. View All» Isotype: IgG View All» Background Information: GSK3 (Glycogen Synthase Kinase 3) differs from most serine/threonine kinases in that it is active in the absence of the action of signaling pathways. Two isoforms exist, GSK3α and GSK3β. The function of GSK3 is to phosphorylate Glycogen Synthase and thereby inactivate it. Insulin action stimulates the PI3 Kinase pathway, resulting in Akt activation, which phosphorylates and inactivates GSK3. Glycogen Synthase is then rapidly dephosphorylated and activated. Other GSK3 substrates include Jun (on inhibitory sites), and eIF2B. Phosphorylation of Tau by GSK3 may relate to development of Alzheimer's disease. Phosphorylation state-specific antibodies directed against the Akt site (Ser21) on GSK3 are suitable for surrogate assays of the activation state of the pathway. View All» Species Reactivity:

• Rat

• Human

• Mouse

• Bovine
  View All» Species Reactivity Note: Rat, Human, Mouse and Bovine View All» Application Notes: Immunoprecipitation: 12.5 µg/mL dilution of a previous lot was ued in immunoprecipitation on rat brain tissue; immunoprecipitations with tissue extracts other than brain may require higher antibody concentrations.Optimal working dilutions must be determined by the end user.Immunohistochemistry(paraffin):Representative testing from a previous lot.Optimal Staining of GSK3β Polyclonal Antibody: HeLa Cells View All» Control: C2C12 Cell Lysate, Hela cells, rat or mouse brain View All» Quality Assurance: Routinely evaluated by Western Blot on C2C12 lysates.Western Blot Analysis: 1:500 dilution of this lot detected GSK3 BETA on 10 μg of C2C12 lysates. View All» Purification Method: Protein A affinity purified View All» Presentation: Purified polyclonal IgG antibody is supplied in PBS, pH 7.2, 50% glycerol and 0.09% sodium azide. View All» Storage Conditions: Stable at –20 ºC for up to one year from date of receipt. Product may be stored at for 2-4 weeks at 2º-8ºC. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P49841 View All» Entrez Gene Number: NM_002093 View All» Gene Symbol:
  • GSK3B

  • EC 2.7.11.26
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Immunoprecipitation

    • Immunohistochemistry (Paraffin)
      View All» Entrez Gene Summary: Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and nactivating glycogen synthase. Two isoforms, alpha (GSK3A; MIM 606784) and beta, show a high degree of amino acid homology (Stambolic and Woodgett, 1994 [PubMed 7980435]). GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation (Plyte et al., 1992 [PubMed1333807]).[supplied by OMIM]. View All» UniProt Summary: FUNCTION: Participates in the Wnt signaling pathway. Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA. Phosphorylates MUC1 in breast cancer cells, and decreases the interaction of MUC1 with CTNNB1/beta-catenin. CATALYTIC ACTIVITY: ATP + [tau protein] = ADP + [tau protein] phosphate. ENZYME REGULATION: Inhibited when phosphorylated by AKT1. SUBUNIT: Monomer (By similarity). Interacts with CABYR, MUC1, NIN and PRUNE. ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing. View All» Product Name: Anti-GSK-3b Antibody, a.a. 335-349 | 07-1413 View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 50 µg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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