货号：07-1419

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-VEGF165 Antibody | 07-1419 View All» Replaces: 07-1419 is a recommended replacement for AB1442. View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: VEGF is a homodimeric glycoprotein growth factor produced by several cell types. At least four VEGF splice variants have been identified. The recombinant VEGF antigen variant used to generate the polyclonal antibody is VEGF165, and no cross-reactivity has been noted with an extensive panel of cytokines and growth factors. Cross reactivity with other splice variants may occur with certain lysates. View All» Molecular Weight: On western blot, bands at VEGF165 (~16 KD kDa), VEGF121 (~12 KD), and VEGF189 (~19KD). View All» Immunogen: Recombinant human VEGF165 View All» Isotype: IgG View All» Background Information: Vascular Endothelial Growth Factor (VEGF) is a potent mitogen for vascular endothelial cells. It significantly influences vascular permeability and is a strong angiogenic protein. VEGF probably also plays a role in neovasularization under physiological conditions. A potent synergism between VEGF and bFGF in the induction of angiogenesis has been observed. Due to its influences on vascular permeability, VEGF may be involved in altering blood-brain-barrier functions under normal and pathological conditions. View All» Species Reactivity:

• Human

• Mouse

• Rat
  View All» Species Reactivity Note: Human, Rat and Mouse View All» Application Notes: Immunohistochemisty (paraffin): VEGF165 staining in human placenta (syncytotrophoblast), tissue was pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was used at 1:100, using IHC-Select® detection with HRP-DAB. Neutralization: A previous lot of this antibody was used in neutralization assays at a dilution of 10 µg/mL.ELISA: A previous lot of this antibody was used in ELISA at a dilution of 1:100 – 1:1000.Optimal working dilutions must be determined by the end user.Western Blot: A previous lot of this antibody was tested on multiple lysates. See datasheet. View All» Control: PC12 cell lysate View All» Quality Assurance: Routinely evaluated by Western Blot on PC12 lysates.Western Blot Analysis: 1:500-1,000 dilution of this lot detected VEGF165 (~16 kDa) on 10 μg of PC12 lysates. View All» Purification Method: HPLC Purified View All» Presentation: Purified rabbit polyclonal IgG in buffer containing TBS and no preservatives. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. For long term storage, aliquot in convenient undiluted aliquots at -20°CHandling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: P15692 View All» Entrez Gene Number: NM_001025366.1 View All» Gene Symbol:
  • VEGF

  • VEGF-A

  • VPF

  • MGC70609

  • VEGF
    View All» Alternate Names: Vascular permeability factor View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • Immunohistochemistry (Paraffin)

    • Neutralizing

    • ELISA
      View All» Entrez Gene Summary: This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Binds to the VEGFR1/Flt-1 and VEGFR2/Kdr receptors, heparan sulfate and heparin. Neuropilin-1 binds isoforms VEGF-165 and VEGF-145. SUBUNIT: Homodimer; disulfide-linked. Also found as heterodimer with PlGF (By similarity). SUBCELLULAR LOCATION: Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin. ALTERNATIVE PRODUCTS: 10 named isoforms [FASTA] produced by alternative splicing. View All» Product Name: Anti-VEGF165 Antibody | 07-1419 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Rabbit View All»
      © 金山科研平台是专业的授权总代理区域代理经销平台。
      © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
      © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
      © 本文地址：https://www.16ao.com/thread-134733.htm
      © Merck Millipore代理07-1419 Anti-VEGF165 (rabbit polyclonal);已停产，产品报价联系微信jinshanbio