货号：07-1436

品牌：Merck Millipore

规格：50Ug

目录价：￥4592.00

市场价格：￥3903.20

会员价格：￥3673.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-PDGF-AA Antibody | 07-1436 View All» Replaces: 07-1436 is a recommended replacement for AB1486P. View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: Reacts with PDGF-AA View All» Molecular Weight: 24 kDa detected in Western Blot. View All» Immunogen: Highly pure (>98%) recombinant human PDGF-AA. View All» Isotype: IgG View All» Background Information: Platelet-Derived Growth Factor (PDGF) is a dimer of A (17 kDa) and B (14 kDa) chains (AA, AB, or BB). Its receptors are transmembrane receptor tyrosine kinases, PDGF Receptors type A and B. The type A PDGF receptor binds all three PDGFs with high affinity, whereas the type B PDGF receptor is selective for PDGF-BB. PDGF signals as a growth and survival factor, as it is a potent activator of the PI3 Kinase pathway. PDGF signaling appears to play an essential role in the development of tumors of glial origin. View All» Species Reactivity:

• Human

• Mouse
  View All» Species Reactivity Note: Human, Mouse View All» Application Notes: Immunohistochemisty (paraffin): PDGF representative staining pattern/morphology in prostate cancer. Tissue was pretreated with citrate pH 6, antigen retrieval. A previous lot of this antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB.ELISA: 0.5 μg/mL dilution of a previous lot allowed detection (in direct ELISA) of 0.2-0.4 ng/well when human PDGF-AA is used to coat the plate.Neutralization: To yield ½ maiximal inhibition (ND50) of the biological activity of hPDGF-AA (5.0 ng/mL), a concentration of 0.05-0.07 μg/mL of a previous lot of this antibody was required.Optimal working dilutions must be determined by the end user. View All» Control: C2C12 cell lysate View All» Quality Assurance: Routinely evaluated by Western Blot on C2C12 lysates. Western Blot Analysis: 1:500 dilution of this lot detected PDGF-AA on 10 μg of C2C12 lysates. View All» Purification Method: Affinity Purfied View All» Presentation: Purified rabbit polyclonal IgG lyophilized from PBS, pH 7.2. View All» Storage Conditions: The lyophilized antibody is stable at room temperature for at least one month and for up to one year when kept at -20ºC. When reconstituted in sterile water to a concentration of 0.1-1.0 mg/mL, the antibody is stable for at least six weeks at 2-8ºC.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: P04085 View All» Entrez Gene Number: NM_002607.4 View All» Gene Symbol:
  • PDGFA

  • PDGF-1

  • PDGF-A

  • PDGF1
    View All» Alternate Names:
    • Platelet-derived growth factor alpha

    • Platelet-derived growth factor alpha chain

    • Platelet-derived growth factor alpha polypeptide
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunohistochemistry (Paraffin)

      • ELISA

      • Western Blotting

      • Neutralizing
        View All» Entrez Gene Summary: The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer or as a heterodimer with the platelet-derived growth factor beta polypeptide, where the dimers are connected by disulfide bonds. Studies using knockout mice have shown cellular defects in oligodendrocytes, alveolar smooth muscle cells, and Leydig cells in the testis; nockout mice die either as embryos or shortly after birth. Two splice variants have been identified for this gene. [provided by RefSeq]. View All» UniProt Summary: FUNCTION: Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its affinity receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound. SUBUNIT: Antiparallel disulfide-linked dimer of non-identical (A and B) chains. Homodimers of A and B chains are implicated in transformation processes. Interacts with CSPG4. SUBCELLULAR LOCATION: Secreted. ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing. Name Long Isoform ID P04085-1 This is the isoform sequence displayed in this entry. Name Short Isoform ID P04085-2 Features which should be applied to build the isoform sequence: VSP_004602, VSP_004603. DOMAIN: The long form contains a basic insert which acts as a cell retention signal. MISCELLANEOUS: A-A and B-B, as well as A-B, dimers can bind to the PDGF receptor. SIMILARITY: Belongs to the PDGF/VEGF growth factor family. View All» Product Name: Anti-PDGF-AA Antibody | 07-1436 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μL View All» Format: Affinity Purified View All» Host: Rabbit View All»
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