货号：07-1451

品牌：Merck Millipore

规格：

目录价：￥3732.00

市场价格：￥3172.20

会员价格：￥2985.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-PAK1 Antibody | 07-1451 View All» Replaces: AB3844 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes the N-terminus of PAK1 but does not cross-react with PAK2, PAK3, or other PAKs. View All» Molecular Weight: Approx. 62 kDa View All» Epitope: N-terminus View All» Immunogen: KLH-conjugated synthetic peptide corresponding to the N-terminus of human PAK1. View All» Background Information: p21-activated kinase 1 (PAK1) is a member of a family of serine/threonine protein kinase defined by their interaction with the small GTPases, Cdc42 and Rac (1-2). The PAK proteins can be essentially divided into two categories, groups I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK5 and PAK6), based on their structures (3). PAK1 participates in regulating the actin cytoskeleton, focal adhesion contacts, cell motility, apoptosis and transcription. It also promotes the disassembly of stress fibers (4-5). PAK1 also binds PIX (PAK-binding protein), COOL and adapter protein Nck (5). View All» Species Reactivity:

• Human

• Mouse

• Rat

• Monkey
  View All» Species Reactivity Note: Reacts with human, mouse, rat, and monkey. View All» Control: NIH/3T3 cell lysate View All» Quality Assurance: Evaluated by Western Blot in NIH/3T3 cell lysate.Western Blot Analysis: 1:500 dilution of this antibody detected PAK-1 on 10 µg of NIH/3T3 cell lysate. View All» Purification Method: Affinity Purfied View All» Presentation: Purified rabbit polyclonal liquid in 10 mM sodium HEPES, pH 7.5, 150 mM NaCl, 100 μg/mL BSA, with 50% glycerol. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. View All» UniProt Number: Q13153 View All» Entrez Gene Number: NP_002567.3 View All» Gene Symbol:
  • Alpha-PAK

  • EC 2.7.11.1

  • MGC130000

  • MGC130001

  • PAK-1

  • PAKalpha

  • p65-PAK
    View All» Alternate Names:
    • STE20 homolog, yeast

    • p21 protein (Cdc42/Rac)-activated kinase 1

    • p21-activated kinase 1

    • p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)

    • p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternativelt spliced transcript variants encoding different isoforms have been found for this gene. View All» UniProt Summary: FUNCTION: The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.COFACTOR: Magnesium.ENZYME REGULATION: Activated by binding small G proteins. Binding of GTP-bound CDC42 or RAC1 to the autoregulatory region releases monomers from the autoinhibited dimer, enables phosphorylation of Thr-423 and allows the kinase domain to adopt an active structure. Also activated by binding to GTP-bound CDC42, independent of the phosphorylation state of Thr-423. Phosphorylation of Thr-84 by OXSR1 inhibits this activation By similarity. SUBUNIT STRUCTURE: Homodimer in its autoinhibited state. Active as monomer. Interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1. Binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length proteins. Component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and GIT1. Interacts with ARHGEF7. Also interacts with CRIPAK. Interacts with NISCH By similarity. Interacts with SCRIB. SUBCELLULAR LOCATION: Cytoplasm. Cell junction › focal adhesion. Note: Recruited to focal adhesions upon activation. PTM: Autophosphorylated when activated by CDC42/p21 and RAC1. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.Contains 1 CRIB domain.Contains 1 protein kinase domain. View All» Product Name: Anti-PAK1 View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Affinity Purified View All» Host: Rabbit View All»
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