货号：07-1520

品牌：Merck Millipore

规格：100Ug

目录价：￥4438.00

市场价格：￥3772.30

会员价格：￥3550.40

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-Grb10 (Ser501/Ser503) Antibody | 07-1520 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes Grb10 phosphorylated at Ser501/Ser503. View All» Molecular Weight: ~66 kDa observed. Uncharacterized bands detected in some lysates. UniProt describes 4 isoforms produced by alternative splicing: Isoform 1 at 62.026 kDa, Isoform 2 at 60.848 kDa, Isoform 3 at 67.231 kDa, and Isoform 4 at 66.487 kDa. View All» Epitope: Phosphorylated Ser501/Ser503. View All» Immunogen: KLH-conjugated linear peptide corresponding to human Grb10 phosphorylated at Ser501/Ser503. View All» Modifications:

• Phosphorylation

• Phosphorylation
  View All» Background Information: Growth factor receptor-bound protein 10 (Grb10) belongs to a small family of structurally related multi-domain adaptor proteins, which are known to be involved with a diverse array of functions, including cellular growth, metabolism, apoptosis, and cell migration. Grb10 is widely, but not uniformly, expressed in mammalian tissues, and there are various known isoforms of Grb10. It is shown to bind to and positively or negatively mediate signals from a number of activated receptor tyrosine kinases, including the insulin (INSR) and the insulin-like growth factor (IGF1R and IGF2R) receptors, in addition to a variety of growth factor receptors and intracellular molecules, in a context-dependent manner. In one study, mTORC1-mediated Grb10 phosphorylation is demonstrated to lead to feedback inhibition of the phosphatidylinositol-3-kinase (PI3K) and the extracellular signal-regulated, mitogen-activated protein kinase (ERK-MAPK) pathways in HEK 293T cells, in vivo. However, many aspects of the Grb10 adapter function remain unclear. Further research is still required to elucidate and validate the interactions and effects of GRB10 with various cellular targets. View All» Species Reactivity:
  • Mouse

  • Human

  • Pig

  • Rhesus Macaque

  • Horse

  • Primate
    View All» Species Reactivity Note: Demonstrated to react with Mouse. Predicted to react with Human, Porcine, Rhesus Macaque, Equine, and Primate based on 100% sequence homology. Other homologies: Rat (91% sequence homology). View All» Control: Insulin untreated and treated mouse embryonic fibroblast cell lysates View All» Quality Assurance: Evaluated by Western Blot in insulin untreated and treated mouse embryonic fibroblast cell lysates.Western Blot Analysis: 1 µg/mL of this antibody detected Grb10 in 10 µg of insulin untreated and treated mouse embryonic fibroblast cell lysates. View All» Purification Method: Affinity purified View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8°C from date of receipt. View All» UniProt Number: Q13322 View All» Entrez Gene Number: NP_005302 View All» Gene Symbol:
    • GRB10

    • GRBIR

    • KIAA0207
      View All» Alternate Names:
      • growth factor receptor-bound protein 10

      • OTTHUMP00000197988

      • OTTHUMP00000197989

      • OTTHUMP00000197990

      • OTTHUMP00000197991

      • OTTHUMP00000197992

      • OTTHUMP00000197993

      • OTTHUMP00000197994

      • GRB10 adapter protein

      • GRB10 adaptor protein

      • maternally expressed gene 1

      • insulin receptor-binding protein Grb-IR
        View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: The product of this gene belongs to a small family of adapter proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with insulin receptors and insulin-like growth-factor receptors. Overexpression of some isoforms of the encoded protein inhibits tyrosine kinase activity and results in growth suppression. This gene is imprinted in a highly isoform- and tissue-specific manner, with expression observed from the paternal allele in the brain, and from the maternal allele in the placental trophoblasts. Alternatively spliced transcript variants encoding different isoforms have been identified. View All» UniProt Summary: FUNCTION: Adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. Binds to, and suppress signals from, activated receptors tyrosine kinases, including the insulin (INSR) and insulin-like growth factor (IGF1R) receptors. The inhibitory effect can be achieved by 2 mechanisms: interference with the signaling pathway and increased receptor degradation. Delays and reduces AKT1 phosphorylation in response to insulin stimulation. Blocks association between INSR and IRS1 and IRS2 and prevents insulin-stimulated IRS1 and IRS2 tyrosine phosphorylation. Recruits NEDD4 to IGF1R, leading to IGF1R ubiquitination, increased internalization and degradation by both the proteasomal and lysosomal pathways. May play a role in mediating insulin-stimulated ubiquitination of INSR, leading to proteasomal degradation. Negatively regulates Wnt signaling by interacting with LRP6 intracellular portion and interfering with the binding of AXIN1 to LRP6. Positive regulator of the KDR/VEGFR-2 signaling pathway. May inhibit NEDD4-mediated degradation of KDR/VEGFR-2.SUBUNIT STRUCTURE: Interacts with ligand-activated tyrosine kinase receptors, including FGFR1, INSR, IGF1R, MET and PDGFRB in a phosphotyrosine-dependent manner through the SH2 domain By similarity. Poorly binds to the EGFR By similarity. Directly interacts with MAP3K14/NIK and is recruited to the EGFR-ERBB2 complex. Interacts with GIGYF1/PERQ1 and GIGYF2/TNRC15 By similarity. When unphosphorylated, interacts with AKT1 and when phosphorylated with YWHAE/14-3-3 epsilon. Interacts with NEDD4. Interacts with LRP6, thus interfering with the binding of AXIN1 to LRP6 By similarity. Binds to activated NRAS.SUBCELLULAR LOCATION: Cytoplasm. View All» Product Name: Anti-phospho-Grb10 (Ser501/Ser503) View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Affinity Purified View All» Host: Rabbit View All»
        © 金山科研平台是专业的授权总代理区域代理经销平台。
        © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
        © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
        © 本文地址：https://www.16ao.com/thread-134897.htm
        © Merck Millipore代理07-1520 Anti-phospho-Grb10 (Ser501/Ser503) Antibody;store at +2℃ to +8℃，产品报价联系微信jinshanbio