货号：07-1594

品牌：Merck Millipore

规格：400UL

目录价：￥6127.00

市场价格：￥5207.95

会员价格：￥4901.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-Phospho-Dnmt1(Ser714) Antibody | 07-1594 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes human Dnmt1 phosphorylated on Ser714. View All» Molecular Weight: ~184 kDa View All» Epitope: Ser714 View All» Immunogen: The immunogen was a KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Ser714 of human Dnmt1. View All» Modifications: Phosphorylation View All» Background Information: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes may be linked with development of cancer. Three families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of Dnmt1 causes global demethylation of genomic DNA and embryonic lethality. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells. Phosphorylation of Ser714 is induced by EGF, but the role of this modification is unknown. View All» Species Reactivity: Human View All» Species Reactivity Note: Human. Not tested in other species. View All» Application Notes: ELISA:This antibody has been shown by an outside laboratory to be suitable for ELISA (1:1000).Dot blotting:This antibody has been shown by an outside laboratory to be specific for the phospho-Ser714 immunogen peptide, but not the unphosphorylated peptide, by dot blotting (1:500). View All» Control: HeLa nuclear extract or EGF-treated whole HeLa cell extract. View All» Quality Assurance: Western Blot Analysis:1:250 dilution of this antibody detected Dnmt1 on 10 µg HeLa nuclear extract View All» Purification Method: Purified View All» Presentation: Purified rabbit polyclonal in buffer containing 0.09% NaN3. View All» Storage Conditions: Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: P26358 View All» Entrez Gene Number: NP_001370 View All» Gene Symbol:

• AIM

• CXXC9

• DNMT

• Dnmt1

• EC 2.1.1.37

• FLJ16293

• M.HsaI

• MCMT

• MGC104992
  View All» Alternate Names:
  • CXXC finger protein 9

  • CXXC-type zinc finger protein 9

  • DNA (cytosine-5-)-methyltransferase 1

  • DNA MTase HsaI

  • DNA methyltransferase 1

  • DNA methyltransferase HsaI
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • ELISA

    • Dot Blot
      View All» Entrez Gene Summary: DNA (cytosine-5-)-methyltransferase 1 has a role in the establishment and regulation of tissue-specific patterns of methylated cytosine residues. Aberrant methylation patterns are associated with certain human tumors and developmental abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a. View All» UniProt Summary: FUNCTION: Methylates CpG residues. Preferentially methylates hemimethylated DNA. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. Ref.17Catalytic activity S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.SUBUNIT STRUCTURE: Interacts with HDAC1 and with PCNA. Forms a complex with DMAP1 and HDAC2, with direct interaction. Forms also a stable complex with E2F1, BB1 and HDAC1. Binds MBD2 and MBD3. Component of complexes containing SUV39H1. Interacts with DNMT3A and DNMT3B. Interacts with the PRC2/EED-EZH2 complex. Ref.17 Ref.8 Ref.9 Ref.10 Ref.11 Ref.13SUBCELLULAR LOCATION: Nucleus. Ref.13TISSUE SPECIFICITY: Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1. Ref.12INDUCTION: Its abundance is reduced to non detectable levels at the G0 phase of the cell cycle and is dramatically induced upon entrance into the S-phase of the cell cycle.SIMILARITY: Belongs to the C5-methyltransferase family.Contains 2 BAH domains.Contains 1 CXXC-type zinc finger.SEQUENCE CAUTION:The sequence AAD54507.1 differs from that shown. Reason: Erroneous gene model prediction. View All» Product Name: Anti- Phospho-Dnmt1(Ser714) View All» Concentration: 0.25 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Rabbit View All»
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