货号：07-1596

品牌：Merck Millipore

规格：100Ug

目录价：￥6127.00

市场价格：￥5207.95

会员价格：￥4901.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-SIRT3 (C-terminus) Antibody | 07-1596 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes SIRT3. Endogenous levels of this protein vary among cell lines; the protein was easily detected in RAW264.7 and C2C12 cells but only weakly detected in HeLa cells, and not detected in Jurkat, HEK293 or PC3 cells. View All» Molecular Weight: 44 kDa View All» Epitope: C-terminus View All» Immunogen: The immunogen was a peptide corresponding the C-terminal region of human SIRT3. View All» Background Information: SIRT3 is a member of the class I sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. SIRT3 is located in the mitochondria and has been implicated in regulating metabolic processes. Endogenous SIRT3 is a soluble protein located in the mitochondrial matrix. Overexpression of Sirt3 in cultured cells increases respiration and decreases the production of reactive oxygen species. Fasting increases Sirt3 expression in white and brown adipose tissue. A longer form of SIRT3 is present in the nucleus where it exhibits histone deacetylase activity. SIRT3 protects cardiomyocytes from stress mediated cell death due to deacetylation of a nuclear factor, Ku-70. View All» Species Reactivity:

• Mouse

• Human
  View All» Species Reactivity Note: Human, Mouse. Not tested in other species. View All» Application Notes: ELISA:This antibody has been shown by an outside laboratory to be suitable for ELISA (1:1000).Immunohistochemistry:This antibody has been shown by an outside laboratory to be suitable for histochemistry using human hepatocarcinoma tissue (1:50 – 1:100). View All» Control: C2C12 cell lysate View All» Quality Assurance: Western Blot Analysis:1:250 dilution of this antibody detected SIRT3 on 10 µg of C2C12 lysate. View All» Purification Method: Purified View All» Presentation: Purified rabbit polyclonal in buffer containing 0.09% NaN3. View All» Storage Conditions: Stable for 6 months at 2-8°C from date of receipt. For long term storage, store at -20ºC. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. View All» UniProt Number: Q9NTG7 View All» Entrez Gene Number: NP_036371 View All» Gene Symbol:
  • SIR2L3

  • hSIRT3

  • EC 3.5.1
    View All» Alternate Names:
    • SIR2-like protein 3

    • mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase

    • silent mating type information regulation 2, S.cerevisiae, homolog 3

    • sir2-like 3

    • sirtuin (silent mating type information regulation 2homolog) 3 (S. cerevisiae)

    • sirtuin (silent mating type information regulation 2,S.cerevisiae, homolog) 3

    • sirtuin 3

    • sirtuin type 3
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • ELISA

      • Immunohistochemistry
        View All» Entrez Gene Summary: This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. [provided by RefSeq]. View All» UniProt Summary: FUNCTION:NAD-dependent deacetylase. Despite some ability to deacetylate histones in vitro, it is unlikely in vivo.Catalytic activity NAD+ + an acetylprotein = nicotinamide + O-acetyl-ADP-ribose + a protein. Ref.4 Ref.5Cofactor Binds 1 zinc ion per subunit By similarity.SUBCELLULAR LOCATION:Mitochondrion matrix. Ref.4 Ref.5TISSUE SPECIFICITY: Widely expressed. Ref.5 Ref.1PTM: Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.SEQUENCE SIMILARITY: Belongs to the sirtuin family.Contains 1 deacetylase sirtuin-type domain. View All» Product Name: Anti-SIRT3 (C-terminus) Antibody | 07-1596 View All» Concentration: 0.25 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Purified View All» Host: Rabbit View All»
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