货号：07-213

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-dimethyl-Histone H4 (Arg3) Antibody | 07-213 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Recognizes methylated Histone H4 (Arg3), MW 10 kDa. Addition of 0.1% Tween®-20 in western blot buffers results in the detection of additional proteins at 17 kDa (possibly methylated Histone H2a), 34 kDa and 80 kDa. Specificity demonstrated by ELISA using methylated and unmethylated Histone H4 peptides. The antibody does not detect recombinant Histone H3 purified from E. coli. View All» Molecular Weight: 10 kDa View All» Immunogen: BSA-conjugated synthetic peptide (SG[me2R]GKGGKG-C) corresponding to amino acids 1-9 of Histone H4 with a C-terminal cysteine added to facilitate conjugation. The immunizing sequence is conserved from Tetrahymena to human. View All» Modifications: Methylation View All» Isotype: IgG View All» Background Information: Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). View All» Species Reactivity:

• Human

• Vertebrates

• Invertebrates
  View All» Species Reactivity Note: Human. Broad species cross-reactivity is expected. View All» Application Notes: Methylation/Western Blot Assay: Reported by an independent laboratory to detect Histone H4 methylated in vitro by recombinant PRMT1 and detected by western blot analysis. View All» Control: Acid extracted proteins from HeLa cells. View All» Quality Assurance: Western Blot Analysis: A 1:500-1:2000 dilution of this antibody detected methylated Histone H4 in acid-extracted proteins from HeLa cells. View All» Purification Method: Immunoaffinity chromatography View All» Presentation: Purified rabbit polyclonal IgG in buffer containing 70% storage buffer 0.2 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide, 5 mg/mL BSA and 30% glycerol. View All» Storage Conditions: Stable for 1 year at from date of receipt. View All» UniProt Number: P62805 View All» Entrez Gene Number: NP_778224 View All» Gene Symbol:
  • HIST1H4A

  • H4/J

  • HIST1H4F

  • HIST1H4L

  • H4FN

  • H4FA

  • H4FH

  • HIST1H4H

  • H4F2

  • H4FM

  • H4/A

  • H4FK

  • H4FG

  • HIST2H4

  • H4FB

  • H4/K

  • HIST1H4I

  • H4/N

  • HIST1H4B

  • H4FE

  • H4/M

  • H4/a

  • HIST1H4E

  • HIST4H4

  • HIST2H4A

  • H4FC

  • H4FI

  • H4/H

  • HIST1H4C

  • H4/G

  • HIST1H4K

  • H4FJ

  • H4FD

  • H4/I

  • H4/B

  • H4/D

  • H4/C HIST1H4J

  • HIST1H4D

  • H4/E
    View All» Alternate Names:
    • H4 histone family, member A

    • histone 1, H4a

    • histone cluster 1, H4a
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications: Western Blotting View All» Entrez Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. View All» UniProt Summary: FUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.SIZE: 103 amino acids; 11367 Da SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.SUBCELLULAR LOCATION: Nucleus.PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family. View All» Brand Family: Upstate View All» Product Name: Anti-dimethyl-Histone H4 (Arg3) Antibody | 07-213 View All» Concentration: Variable View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Affinity Purified View All» Host: Rabbit View All»
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