货号：07-329

品牌：Merck Millipore

规格：100UL

目录价：￥6742.00

市场价格：￥5730.70

会员价格：￥5393.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-acetyl-Histone H4 (Lys16) Antibody | 07-329 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Histone H4 acetylated on lysine 16, but not histone H4 containing a lysine 16 to arginine substitution. View All» Molecular Weight: 10 kDa View All» Epitope: Lys16 View All» Immunogen: Ovalbumin-conjugated, synthetic peptide (…GAAcKRH …) in which AcK corresponds to residue 16 of yeast histone H4. View All» Modifications: Acetylation View All» Isotype: IgG View All» Background Information: Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Acetylation of histone H4 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). View All» Species Reactivity:

• Human

• Vertebrates

• Yeast (S. cerevisiae)
  View All» Species Reactivity Note: Yeast and human. Broad species cross-reactivity is expected. View All» Application Notes: Multiplexing: This antibody specifically recognizes histone H4 acetylated on Lys16 by Luminex® assay.Peptide Inhibition Assay: This antibody peptide blocked on HeLa cell extracts.Chromatin Immunoprecipitation An independent laboratory has shown that this antibody preferentially immunopre-cipitates chromatin from wild type yeast, compared to yeast containing a histone H4 lysine 16 to arginine substitution. View All» Control:
  • TSA treated NIH/3T3 cells.

  • HeLa acid extract
    View All» Quality Assurance: Evaluated by western blot on acid extracts of HeLa cells.Western Blot Analysis:0.1 µg/mL of this antibody detected Histone H4 acetylated on Lys16 in 10 µg of HeLa Acid extract cell lysate. View All» Purification Method: Affinity Purfied View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4) 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Stable for 1 year at 2-8° C from date of receipt.For maximum recovery of product, centrifuge the vial prior to removing the cap. View All» UniProt Number: P62805 View All» Entrez Gene Number: NM_175054 View All» Gene Symbol:
    • HIST1H4A

    • H4/J

    • HIST1H4F

    • HIST1H4L

    • H4FN

    • H4FA

    • H4FH

    • HIST1H4H

    • H4F2

    • H4FM

    • H4/A

    • H4FK

    • H4FG

    • HIST2H4

    • H4FB

    • H4/K

    • HIST1H4I

    • H4/N

    • HIST1H4B

    • H4FE

    • H4/M

    • H4/a

    • HIST1H4E

    • HIST4H4

    • HIST2H4A

    • H4FC

    • H4FI

    • H4/H

    • HIST1H4C

    • H4/G

    • HIST1H4K

    • H4FJ

    • H4FD

    • H4/I

    • H4/B

    • H4/D

    • H4/C

    • HIST1H4J

    • HIST1H4D

    • H4/E
      View All» Alternate Names:
      • H4K16Ac

      • Histone H4 (acetyl K16)

      • H4 histone family, member A

      • histone 1, H4a

      • histone cluster 1, H4a
        View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
        • Western Blotting

        • Multiplexing

        • Peptide Inhibition Assay

        • Dot Blot

        • ChIP-seq
          View All» Entrez Gene Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. View All» UniProt Summary: FUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.SIZE: 103 amino acids; 11367 Da SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.SUBCELLULAR LOCATION: Nucleus.PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family. View All» Brand Family: Upstate View All» Product Name: Anti-acetyl-Histone H4 (Lys16) Antibody | 07-329 View All» Concentration: Variable View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 µL View All» Format: Affinity Purified View All» Host: Rabbit View All»
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