金山科研平台，产品价格货期咨询微信：jinshanbio Source:Mouse myeloma cell line, NS0-derived, Asp20-Cys561, with a C-terminal 6-His tag Accession #:AAH68308 N-terminal Sequence Analysis:Asp20 Purity:>90%, by SDS-PAGE under reducing conditions and visualized by silver stain. Predicted Molecular Mass:60.3 kDa SDS-PAGE:80-95 kDa, reducing conditions Activity:Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells.When 5 x 10⁴ cells/well are added to rmNephronectin coated plates (10 µg/mL, 100 µL/well), approximately 50 - 80% will adhere after 1 hour at 37 °C. Formulation:Lyophilized from a 0.2 µm filtered solution in PBS.See Certificate of Analysis for details. Molecule Information: Nephronectin Aliases: EGFL6L; Nctn; NPNT; POEM Entrez Gene IDs: 255743 (Human); 114249 (Mouse) Background: Nephronectin

Nephronectin, also called POEM (preosteoblast EGF repeat protein with MAM domain) is a 70 - 90 kDa extracellular matrix protein that is a ligand for integrin alpha8beta1. Nephronectin is most highly expressed in developing endocrine organs such as parathyroid, thyroid, hypophysis and pineal organ, and around developing bone, teeth, and muscle. Pre-osteoblast cells produce nephronectin, but downregulate it as they differentiate. It is also expressed in the Wolffian duct and ureteric bud basement membranes in the developing kidney, where it is colocalizes, and forms an in vivo complex with integrin alpha8beta1. Since deletion of integrin alpha8beta1 results in renal agenesis, nephronectin has been proposed to be a critical integrin alpha8beta1 ligand during nephrogenesis.

The 561 aa mouse nephronectin (isoform B) contains a 19 amino acid (aa) signal sequence, a matrilin-type vWA domain, two calcium-binding EGF-like domains, a potential N-linked glycosylation site, a pro/ser/thr-rich mucin-like region, an RGD integrin binding motif, and a MAM domain. These features are often present in matrix proteins that, like nephronectin, mediate cell adhesion and spreading. Isoform A includes a 17 aa insertion between aa # 58 and 59 that is missing in Nephronectin isoform B. Mouse nephronectin shows 88%, 92%, 85% and 77% aa identity with human, rat, cow and opossum nephronectin, respectively.