R&D Systems代理441-OP-200 Recombinant Mouse Osteopontin (OPN) Protein (200 UG)-试剂-金山科研平台

R&D Systems代理441-OP-200 Recombinant Mouse Osteopontin (OPN) Protein (200 UG)

2025-06-27

货号：441-OP-200

品牌：R&D Systems

规格：200ug

目录价：￥9540.00

市场价格：￥7632.00

会员价格：￥7632.00

到货时间：3~4周

金山科研平台，产品价格货期咨询微信：jinshanbio Source:Mouse myeloma cell line, NS0-derived, Leu17-Asn294 (Glu99Gly), with a C-terminal 6-His tag Accession #:Q547B5 N-terminal Sequence Analysis:Leu17 Purity:>95%, by SDS-PAGE under reducing conditions and visualized by silver stain. Predicted Molecular Mass:31.5 kDa SDS-PAGE:30 kDa and a 65 kDa doublet, reducing conditions Activity:Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. Agnihotri, R. et al. (2001) J. Biol. Chem. 276:28261.

Recombinant Mouse (rm)Osteopontin/OPN immobilized at 2 µg/mL, 100 µL/well, will mediate >25% 293 cell adhesion (1 x 10⁵ cells/well). Recombinant Human Coagulation Factor II/Thrombin (Catalog# 1473-SE) proteolytic treatment of this rmOsteopontin can increase 293 cell adhesion by about 10-fold.

Molecule Information: Osteopontin/OPN Long Name: Secreted Phosphoprotein 1 [BNSP] Aliases: Eta-1; OPN; Spp1 Entrez Gene IDs: 6696 (Human); 20750 (Mouse); 25353 (Rat); 281499 (Bovine) Background: Osteopontin (OPN) View Osteopontin IHC images. Osteopontin (OPN), previously referred to as transformation-associated secreted phosphoprotein, bone sialoprotein I, and minopotin, is a secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein originally isolated from bone matrix. Subsequently, OPN has been found in kidney, placenta, blood vessels, and various tumor tissues. Human, mouse, rat, porcine, and bovine OPN share approximately 40% amino acid sequence identity.

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