货号：07-672

品牌：Merck Millipore

规格：EA

目录价：询价

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-MYPT1 Antibody | 07-672 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Recognizes MYPT1, MW ~120-130 kDa. View All» Molecular Weight: ~120-130 kDa View All» Immunogen: MBP fusion protein corresponding to amino acids 714-1004 of chicken myosin phosphatase targeting subunit 1 (MYPT1). View All» Isotype: IgG View All» Background Information: Myosin phosphatase target subunit 1 (MYPT1), also called Myosin-binding subunit of Myosin phosphatase, is one of the subunits and an integral component of the Myosin phosphatase. Myosin phosphatase regulates the interaction of Actin and Myosin downstream of the guanosine triphosphatase Rho, which inhibits Myosin phosphatase through the action of Rho-kinase. It is also involved in smooth muscle contraction. Inhibition of Myosin light chain phosphatase results in Ca2+ sensitization of smooth muscle contraction. This inhibition is modulated through phosphorylation of MYPT1 by a ZIP-like kinase, which associates with MYPTI and phosphorylates the inhibitory site in smooth muscle. The phosphorylation of MYPT1 by protein kinase C results in altered dephosphoryation of Myosin by attenuating the binding of protein phosphatase 1 catalytic subunit (PP1c) and the phosphorylated 20 kDa Myosin light chain to MYPT1. PP1c interacts with at least four binding sites on the amino-terminus of MYPT1. MYPT1 is localized on stress fibers, and is distributed close to the cell membrane and at cell-cell contacts to regulate Myosin phosphatase activity. View All» Species Reactivity:

• Human

• Chicken

• Vertebrates
  View All» Species Reactivity Note: Human and chicken. Broad species cross-reactivity is expected due to sequence homology. View All» Application Notes: Immunoprecipitation: 2 µg of a previous lot immunoprecipitated MYPT1 from 400 µg of HeLa cell lysate. View All» Control: HeLa cell lysate View All» Quality Assurance: Evaluated by western blot in HeLa cell lysates.Western Blot Analysis: 0.1-2 µg/mL of this antibody detected MYPT1 in HeLa cell lysates. View All» Purification Method: Protein A purfied View All» Presentation: Purified rabbit polyclonal IgG in buffer containing 70% storage buffer 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol. View All» Storage Conditions: Stable for 1 year at -20ºC from date of receipt. View All» UniProt Number: O14974 View All» Entrez Gene Number: NP_002471 View All» Gene Symbol:
  • PPP1R12A

  • MGC133042

  • MBS

  • MYPT1
    View All» Alternate Names:
    • Myosin phosphatase target subunit 1

    • Myosin phosphatase-targeting subunit 1

    • Protein phosphatase myosin-binding subunit

    • myosin phosphatase, target subunit 1

    • protein phosphatase 1, regulatory (inhibitor) subunit 12A
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Immunoprecipitation

      • Western Blotting
        View All» Entrez Gene Summary: Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. View All» UniProt Summary: FUNCTION: SwissProt: O14974 # Regulates myosin phosphatase activity.SIZE: 1030 amino acids; 115281 Da SUBUNIT: PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12A mediates binding to myosin. Interacts with ARHA and CIT (By similarity). Binds PPP1R12B, ROCK1 and IL16.SUBCELLULAR LOCATION: Cytoplasm. Note=Along actomyosin filaments and stress fibers.PTM: Phosphorylated by CIT (Rho-associated kinase) (By similarity). Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.SIMILARITY: SwissProt: O14974 ## Contains 6 ANK repeats. View All» Brand Family: Upstate View All» Product Name: Anti-MYPT1 Antibody | 07-672 View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 200 μg View All» Format: Purified View All» Host: Rabbit View All»
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