货号：07-729

品牌：Merck Millipore

规格：200Ul

目录价：￥6742.00

市场价格：￥5730.70

会员价格：￥5393.60

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-CTCF Antibody | 07-729 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Trade Name: Upstate (Millipore) View All» Specificity: Recognizes CTCF at a.a. 659-675. View All» Molecular Weight: Approx. 120 kDa View All» Immunogen: KLH-conjugated, synthetic peptide corresponding to amino acids 659-675 (C-TNQPKQNQPTAIIQVED) of human CCCTC-binding factor (CTCF) with a N-terminal cysteine added for conjugation purposes. View All» Background Information: CTCF is a ubiquitous 11 zinc finger (ZF) protein with highly versatile functions. A transcriptional repressor, CTCF, binds to promoters of vertebrate c-myc gene. It also binds to the PLK and PIM1 promoters. CTCF may prevent the access of transcriptional activators to enhancers. CTCF also acts as a transcriptional activator of APP. It is involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation-dependent chromatin insulation, and genomic imprinting. CTCF may also act as tumor suppressor. CTCF organizes epigenetically controlled chromatin insulators that regulate imprinted genes in soma. View All» Species Reactivity:

• Human

• Rat

• Canine

• Mouse

• Primate
  View All» Species Reactivity Note: Human. Predicted to cross-react with mouse, rat, dog and primates based on sequence homology. View All» Application Notes: Chromatin Immunoprecipitation: An independent laboratory has confirmed that this antibody immunoprecipitates CTCF-bound chromatin from WEHI B-cells. View All» Control: HeLa nuclear extract.Included Positive Antigen Control: Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 µL of extract and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced extract per lane for minigels. View All» Quality Assurance: Routinely evaluated by Western Blot.Western Blot Analysis: A 1:1000–1:5000 dilution of this lot detected CTCF in HeLa nuclear extract. A previous lot detected CTCF in K562 nuclear extract (data not shown). View All» Purification Method: Unpurified View All» Presentation: Rabbit polyclonal antiserum containing 0.05% sodium azide and 30% glycerol. View All» Storage Conditions: Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage. View All» UniProt Number: P49711 View All» Entrez Gene Number: NM_006565.2 View All» Gene Symbol: CTCF View All» Alternate Names:
  • 11 zinc finger transcriptional repressor

  • 11-zinc finger protein

  • CCCTC-binding factor

  • CCCTC-binding factor (zinc finger protein)

  • CTCFL paralog

  • transcriptional repressor CTCF
    View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
    • Western Blotting

    • ChIP-seq
      View All» Entrez Gene Summary: This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. View All» UniProt Summary: FUNCTION:SwissProt: P49711 # Transcriptional repressor binding to promoters of vertebrate c-myc gene. Also binds to the PLK and PIM1 promoters. May prevent the access of transcriptional activators to enhancers. Also acts as a transcriptional activator of APP. Involved in different aspects of gene regulation including promoter activation or repression, hormone-responsive gene silencing, methylation- dependent chromatin insulation, and genomic imprinting. Seems to act as tumor suppressor.SIZE: 727 amino acids; 82785 Da SUBCELLULAR LOCATION: Nucleus.TISSUE SPECIFICITY:Ubiquitous. Absent in primary spermatocytes.SIMILARITY: SwissProt: P49711 ## Belongs to the CTCF zinc-finger protein family. & Contains 11 C2H2-type zinc fingers. View All» Brand Family: Upstate View All» Product Name: Anti-CTCF Antibody | 07-729 View All» Concentration: Variable View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 200 µL View All» Format: Serum View All» Host: Rabbit View All»
      © 金山科研平台是专业的授权总代理区域代理经销平台。
      © 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
      © 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
      © 本文地址：https://www.16ao.com/thread-136257.htm
      © Merck Millipore代理07-729 Anti-CTCF Antibody;抗CTCF抗体store at -20℃，产品报价联系微信jinshanbio