金山科研平台，产品价格货期咨询微信：jinshanbio Source:Chinese Hamster Ovary cell line, CHO-derived, Ser19-Lys352 Accession #:NP_149122 N-terminal Sequence Analysis:Ser19 Purity:>75%, by SDS-PAGE with silver staining. Endotoxin Level: Predicted Molecular Mass:37.4 kDa SDS-PAGE:40 kDa, reducing conditions Activity:Measured by its ability to induce alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED₅₀ for this effect is typically 5-25 ng/mL. Measured by its ability to induce activation of beta -catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED₅₀ for this effect is typically <500 ng/mL.Optimal concentrations should be determined by each laboratory for each application.Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line. Molecule Information: Wnt-3a Long Name: Wingless-type MMTV Integration Site Family, Member 3a Entrez Gene IDs: 89780 (Human); 22416 (Mouse) Background: Wnt-3a

Wnt-3a is one of about 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved, cysteine-rich secreted glycoproteins important for normal developmental processes. Wnts bind to receptors of the Frizzled family in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6), or the Ryk atypical receptor tyrosine kinase. During development, Wnt-3a is a morphogen that is thought to coordinate somitogenesis and mesoderm boundary determination. When Wnt-3a is deleted, mice fail to develop a hippocampus, and show defects in anterior-posterior patterning, somite development and tailbud formation. Wnt-3a has also been implicated in chondrocyte differentiation.

Like other Wnts, Wnt-3a is modified by palmitate addition (at Cys 77) following glycosylation, which increases its hydrophobicity, secretion and activity. A second site at Ser 209 modified by palmitoleic acid also contributes. Human Wnt-3a shares 96% amino acid (aa) identity with mouse, bovine and canine Wnt-3a, and 89%, 86% and 84% aa identity with chicken, Xenopus and zebrafish Wnt-3a, respectively. It also shares 87% aa identity with Wnt-3. Human Wnt-3a is a 44 kDa secreted hydrophobic glycoprotein containing a conserved pattern of 24 cysteine residues.