货号：09-290

品牌：Merck Millipore

规格：

目录价：￥4239.00

市场价格：￥3603.15

会员价格：￥3391.20

金山科研平台，产品价格货期咨询微信：jinshanbio Description: Anti-phospho-AMPK1 (Thr174) Antibody | 09-290 View All» Promotional Text: Special Shipping Offer on Antibodies100% Performance Guaranteed View All» Specificity: This antibody recognizes phosphorylated AMPK1 at Thr174. View All» Molecular Weight: 60 kDa View All» Immunogen: KLH-conjugated linear peptide corresponding to phosphorylated human AMPK1 (Thr174). View All» Modifications: Phosphorylation View All» Background Information: AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostasis, and a key target for treating Type 2 diabetes and obesity. The Alpha subunit has at least two isoforms (alpha 1 and alpha 2) that differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 174 in the activation loop of the alpha subunit is a key determinant of AMPK activity. View All» Species Reactivity:

• Human

• Rat

• Mouse

• Canine

• Bovine

• Chicken
  View All» Species Reactivity Note: Reacts with human. Predicted to react with mouse, rat, dog, cow, and chicken based on sequence homology. View All» Application Notes: Western Blot (SNAP ID) Analysis: 2 µg/mL of this antibody detected AMPK1 phosphorylated at Thr174 in HEK293 cell lysate (see page 2). View All» Control: Lambda phosphatase treated and untreated Hek293 cell lysate View All» Quality Assurance: Evaluated by Western Blot in Lambda phosphatase treated and untreated HEK293 cell lysate.Western Blot Analysis: 2 µg/mL of this antibody detected AMPK1 phosphorylated at Thr174 in Lambda phosphatase treated and untreated HEK293 cell lysate. View All» Purification Method: Affinity Purfied View All» Presentation: Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 0.05% sodium azide. View All» Storage Conditions: Maintain refrigerated at 2-8°C for up to 12 months. View All» UniProt Number: Q13131 View All» Entrez Gene Number: NP_006242 View All» Gene Symbol:
  • AMPK

  • AMPK1

  • AMPKa1

  • EC 2.7.11.1

  • MGC33776

  • MGC57364
    View All» Alternate Names:
    • 5'-AMP-activated protein kinase, catalytic alpha-1 chain

    • AMP -activate kinase alpha 1 subunit

    • AMP-activated protein kinase, catalytic, alpha-1

    • AMPK alpha 1

    • AMPK alpha-1 chain

    • protein kinase, AMP-activated, alpha 1 catalytic subunit
      View All» Usage Statement: Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. View All» Key Applications:
      • Western Blotting

      • Immunohistochemistry
        View All» Entrez Gene Summary: The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. View All» UniProt Summary: FUNCTION: Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.COFACTOR: Magnesium.ENZYME REGULATION: Binding of AMP results in allosteric activation, inducing phosphorylation on Thr-183 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Also activated by phosphorylation by CAMKK2 triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio. SUBUNIT STRUCTURE: Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic subunits. Interacts with FNIP1 and FNIP2. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.Contains 1 protein kinase domain.SEQUENCE CAUTION: The sequence AAH48980.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened. View All» Product Name: Anti-phospho-AMPK1 (Thr174) View All» Concentration: 1 mg/mL View All» Antibody Type: Polyclonal Antibody View All» Qty/Pk: 100 μg View All» Format: Affinity Purified View All» Host: Rabbit View All»
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