金山科研平台，产品价格货期咨询微信：jinshanbio Source:Chinese Hamster Ovary cell line, CHO-derived, Ser27-Ser395, with an N-terminal 6-His tag Accession #:NP_064334 N-terminal Sequence Analysis:His Purity:>95%, by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin Level: Predicted Molecular Mass:42 kDa SDS-PAGE:57 kDa, reducing conditions Activity:Measured by its ability to transfer sulfate from PAPS to N-acetyl-D-glucosamine.The specific activity is >300 pmol/min/µg, as measured under the described conditions. Formulation:Supplied as a 0.2 µm filtered solution in Tris and NaCl.See Certificate of Analysis for details. Molecule Information: Carbohydrate Sulfotransferase 5/CHST5 Aliases: CHST5; GlcNAc6ST3; GST4-alpha; hIGn6ST; I-GlcNAc6ST Entrez Gene IDs: 23563 (Human); 56773 (Mouse); 307859 (Rat) Background: Carbohydrate Sulfotransferase 5 The CHST family is comprised of 14 genes in both the human and mouse genomes. All members of this family are Golgi-localized type II membrane proteins. Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids. This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses. CHST5, also designated as I-GlcNAc6ST, GlcNAc6ST-3, and GST-4 alpha, has a marked preference for sulfating O-linked sugars of mucin type of acceptors and catalyzes the transfer of sulfate to position 6 of non-reducing GlcNAc residues on keratan sulfate. The amino acid sequence for mouse CHST5 has higher identity to that of human CHST6 (83%) than to human CHST5 (76%). Since there is no CHST6 found in the mouse genome it is possible that mouse CHST5 plays a similar biological role to the human CHST6.