金山科研平台，产品价格货期咨询微信：jinshanbio Source:Chinese Hamster Ovary cell line, CHO-derived, Val21-His428 (major) & Ser46-His428 (minor), both with a C-terminal 6-His tag Accession #:O75356 N-terminal Sequence Analysis:Val21 & Ser46 Purity:>90%, by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin Level: Predicted Molecular Mass:46 kDa SDS-PAGE:50-60 kDa, reducing conditions Activity:Measured by its ability to hydrolyze the 5’-phosphate groups from the substrate guanosine-5’-diphosphate (GDP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit (Catalog# DY996).The specific activity is >7,000pmol/min/µg, as measured under the described conditions. Formulation:Supplied as a 0.2 µm filtered solution in Tris, CaCl₂, NaCl and Glycerol.See Certificate of Analysis for details. Molecule Information: CD39L4/ENTPD5 Long Name: Ecto-Nucleoside Triphosphate Diphosphohydrolase 5 Aliases: CD39L4; ENTPD5; ER-UDPase; NTPDase 5; PCPH Entrez Gene IDs: 957 (Human); 12499 (Mouse); 314312 (Rat) Background: CD39L4 CD39L4 (also ER-UDPase, PCPH and Ectonucleoside triphosphate diphosphohydrolase 5/eNTPDase5) is a secreted, 45-51 kDa class II member of the GDA1/CD39 NTPase family of molecules. It is expressed by multiple cell types, including neurons, cardiomyocytes, and hepatocytes. CD39L4 is a divalent cation-dependent enzyme that preferentially hydrolyzes GDP and UDP. In the ER, this enzyme may remove UDP which interferes with proper protein folding and maturation. Extracellularly, CD39L4 may reduce available UDP ligand for P2Y receptors. Mature human CD39L4 is a 408 amino acid (aa) glycoprotein (aa 21-428). It contains four APR (apyrase conserved regions) (aa 54-206) that participate in NTPase activity. CD39L4 has been reported to exist as both monomer and homodimer. Three potential splice variants are reported. One shows a seven aa substitution for aa 401-428, a second contains a five aa substitution for aa 401-428, and a third possesses a 22 aa substitution for aa 215-428. Over aa 21-428, human CD39L4 shares 89% aa identity with mouse CD39L4.