金山科研平台，产品价格货期咨询微信：jinshanbio Source:Spodoptera frugiperda, Sf 21 (baculovirus)-derived, Asn2-Pro654, with an N-terminal 5-His tag Accession #:Q9NRA0 N-terminal Sequence Analysis:His Purity:>90%, by SDS-PAGE under reducing conditions and visualized by silver stain. Endotoxin Level: Predicted Molecular Mass:70 kDa SDS-PAGE:70-85 kDa, reducing conditions Activity:Measured by its ability to phosphorylate Omega-(7-nitro-2-1,3-benzoxadiazol-4-yl)-D-erythro-sphingosine (NBD-sphingosine).The specific activity is >1,500 pmol/min/µg, as measured under the described conditions. Formulation:Supplied as a 0.2 µm filtered solution in MES, NaCl, Glycerol and DTT.See Certificate of Analysis for details. Molecule Information: Sphingosine Kinase 2/SPHK2 Aliases: SK2; SPK2 Entrez Gene IDs: 56848 (Human); 56632 (Mouse); 308589 (Rat) Background: Sphingosine Kinase 2/SPHK2 Sphingosine Kinase 2 (SPHK2) is one of two enzymes that catalyze the phosphorylation of Sphingosine to form Sphingosine 1-phosphate (S1P). SPHK2 is 654 amino acids (aa) in length with a predicted molecular mass of 69 kDa. The mouse and rat orthologs share 84% and 85% aa sequence identity with the human protein, respectively. Three human SPHK2 splice variants have been identified. While both SPHK1 and SPHK2 generate S1P, the effects of SPHK2 signaling oppose those of SPHK1. SPHK1 activation results in cell proliferation and prevents apoptosis, and SPHK2 activation inhibits cell proliferation and promotes apoptosis. SPHK1 is primarily localized to the cytoplasm and plasma membrane, while SPHK2 is primarily localized to the endoplasmic reticulum. These differences in their localization may contribute to the differences in their signaling. As SPHK activity affects a wide range of biological processes including development, the immune response, and tumorigenesis, SPHK inhibition is a therapeutic target of interest.